摘要
目的:观察复方丹参滴丸对细菌内毒素(LPS)所致人血管内皮细胞功能及形态结构损伤的作用。方法:人脐静脉内皮细胞培养,建立脂多糖损伤模型(以10 mg/L的LPS培养液培养细胞12 h),按分组分别把不同浓度的丹参滴丸(1 g/L、0.5 g/L、0.25 g/L、0.1 g/L)于损伤前、损伤后加入,分别进行细胞活力、一氧化氮(NO)、一氧化氮合酶(NOS)、内皮素(ET-1)、细胞内钙浓度的测定及倒置显微镜、倒置相差显微镜、激光共聚焦显微镜、透射电镜等形态学指标观察。结果:损伤后给不同浓度丹参滴丸对LPS所致人血管内皮细胞损伤均有明显减轻(P<0.05)。其中0.5 g/L作用最为突出(P<0.01)。损伤前给药只有浓度0.5 g/L、0.25 g/L对LPS所致内皮细胞损伤有明显减轻(P<0.05),而高浓度(1 g/L)、低浓度(0.1 g/L)虽亦有减轻,但无统计学意义(P>0.05)。损伤前、后分别给予不同浓度滴丸后,LPS所致血管内皮细胞形态结构损伤均有明显减轻。结论:丹参滴丸对血管内皮细胞具明显保护作用。
AIM： To observe the function and morphological change of human umbilical vein endothelial cells （HU- VECs） treated with lipopolysaccharide （LPS） and composite salviae dropping pills （DSP）. METHODS： HUVECs were cultured and incubated within 10 mg/L LPS for 12 hours. Different final concentrations of composite salviae dropping pills （1 g/L, 0.5 g/L, 0.25 g/L, 0.1 g/L） were added before and after LPS treatment. Cell viability, NO, NOS, ET- 1 and intracellular calcium were measured. The cells were observed under inverted microscope, inverted phase contrast microscope, laser confncal scanning microscopy and transmission electron microscope. RESULTS： When given after LPS treatment, different final concentrations of composite salviae dropping pills played a protective role （ P 〈 0.05）, and the concentration of 0.5 g/L was the most effective （ P 〈 0.01）. When given before LPS treatment, 0.5 g/L and 0.25 g/L of composite salviae dropping pills protected the human vascular endothelial cells （ P 〈 0.05）, but 1 g/L and 0.1 g/L didn＇t play a protective role （ P 〉 0.05）. The HUVECs ＂injured by LPS underwent apparent morphological change after treatment with composite salviae dropping pills. CONCLUSION; Composite salviae dropping pills have an evident protective effect on human umbilical vein endothelial cells exposed to LPS.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第5期933-937,共5页
Chinese Journal of Pathophysiology
