摘要
目的:探讨乌司他丁对急性坏死性胰腺炎大鼠核因子-κ B(NF-κB)的表达及有关细胞因子的影响．方法:将30只Sprague-Dawley大鼠随机分为假手术组(SO 组)、急性坏死性胰腺炎组(ANP组)和急性坏死性胰腺炎乌司他丁治疗组(UTI组),比较各组大鼠NF-κB、IL-6、 TNF-α、血清淀粉酶的水平．结果:ANP组淀粉酶活性195 907．51±38 618．39 nkat／L, TNF-α值41.37±7．54 ng／L,IL-6值32．32±8．62 ng/L,肺、胰腺组织NF-κB表达增加,与SO组比较P均<0．01,乌司他丁治疗后,淀粉酶活性69 804．30±19 432．55 nkat／L,TNF-α值 18．66±6．45 ng／L,IL-6值23．41±7．65 ng／L,肺、胰腺组织NF- κB表达降低,与SO组和ANP组比较P均<0．01．结论:急性坏死性胰腺炎时NF-κB活化,上调IL-6、TNF-α, 加重胰腺及肺的损伤,乌司他丁抑制NF-κB表达而减轻胰腺及肺的损伤,改善急性坏死性胰腺炎的预后．
AIM： To evaluate the effect of ulinastatin （UTI） on the expression of nuclear factor-κB （NF-κB） and related cytokines in acute necrotic pancreatitis （ANP） in rats. METHODS： Thirty Sprague-Dawley rats were randomly divided into sham operation （SO） group, ANP group and UTI treatment group. The levels of NF-κB, interleukin-6 （IL-6）, tumor necrosis factor-α （TNF-α） and serum amylase in rats were detected. ANP model was induced by retrograde administration of 50 g/L sodium taurocholate into biliary-pancreatic duct. RESULTS： In ANP group, the levels of serum amylase （195 907.51±38 618.39 nkat/L）, TNF-α （41.37±7.54 ng/L）, IL-6 （32.32±8.62 ng/L） as well as the expression of pancreatic and pulmonary NF-κB were all increased as compared with those in SO group （all P 〈0.01）. After treatment with UTI, the levels of serum amylase （69 804.30± 19 432.55 nkat/L）, TNF-α （18.66±6.45 ng/L）, IL-6 （23.41±7.65 ng/L） as well as the expression of pancreatic and pulmonary NF-κB were all significantly decreased as compared with those in SO and ANP group （P 〈0.01）. CONCLUSION： In the pathogenesis of ANP, activated NF-κB and up-regulated TNF-α and IL-6 aggravated the injuries of pancreas and lung tissues. UTI plays a protective role in ANP by inhibiting the activation of NF-κB, so as to alleviate the injuries of pancreas and lung tissues.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第22期2700-2703,共4页
World Chinese Journal of Digestology
