摘要
目的:研究к阿片受体选择性激动剂(U50,488H)和肾上腺素受体激动剂(去甲肾上腺素NE)对大鼠离体心脏缺血再灌注(ischemiaandreperfusion,IR)室性心律失常的影响.方法:将35只SD大鼠完全随机设计分为五组(每组7只):假手术组;单纯缺血再灌注(I/R)组;I/R+NE组;I/R+U50组;I/R+U50+NE组.分离正常大鼠心脏,采用Langendorff离体心脏灌流方法,结扎冠脉前降支20min,再灌20min造成IR.在此期间分别应用U50,488H(5×10-6mol/L)和NE(1×10-7mol/L)进行干预.并测定心脏功能的相关指标.结果:①心脏缺血后,心脏各项生理功能指标明显下降,心率上升,再灌注后情况有所恢复.与IR组相比较,给予U50,488H后,心脏各项生理功能指标变化不明显,心率明显下降(P<0.05).②假手术对照组偶发早搏,IR组心律失常发生频率明显增加,与IR组相比较,IR+NE组心律失常评分明显增高(P<0.01);如果提前给予U50,488H,发现不仅可以明显降低IR组缺血和再灌注期间室速和室颤的发生率(P<0.01),和降低IR组心律失常的评分,而且明显降低IR+NE组心律失常的评分(P<0.01).结论:κ阿片受体激动剂U50,488H对于NE诱导的缺血再灌注时心律失常有拮抗作用.
AIM： To investigate the effect of U50, 488H （a selective κ-opioid receptor agonist） and noradrenalin （ NE, an adrenergic receptor agonist） on ventricular arrhythmias induced by myocardial ischemia and reperfusion in rats in vitro. METHODS： Rats were randomly divided into 5 groups （7 rats in each group）, i. e., normal control group, ischemia/reperfusion group （I/R）, U50,488H ＋ I/R group, NE ＋ I/R group, U50, 488H ＋ NE ＋ I/R group. The isolated heart was perfused by using Langendorff equipment and the front branch of coronary artery was ligated for 20 min, and then the perfusion was resumed for 20 min to engender the I/R condition. U50,488H（5 × 10^-6 tool/L） and NE（ 1 × 10^-7 tool/L） were used to interfere the effects during myocardial ischemia and reperfusion. Heart rate （HR）, arterial blood pressure （ABP）, left ventricular pressure （LVP）, cardiac function （ ＋ dp/dtmax） , rate of ventricular tachycardia （VT） and ventricular fibrillation （VF） were determined in rats. RESULTS： ① Every index of the heart functions decreased significantly, and HR increased after myocardial ischemia. With myocardial reperfusion, the condition was improved a little. Compared with I/R group, HR decreased significantly with U50, 488H administration （ P 〈 0. 05 ）.② Compared with normal control group and I/R group, the incidence of ventricular arrhythmias and arrhythmia score were enhanced in I/R ＋ NE group （P 〈0.01 ）. However, it showed that not only the incidence of VT and VF score during myocardial ischemia and reperfusion and ventricular arrhythmias and arrhythmia score of I/R group, but also ventricular arrhythmias and arrhythmia score of I/R ＋ NE group decreased significantly with administration of US0,488H （P 〈 0.01 ）. CONCLUSION ： κ-opioid receptor agonist U50, 488H antagonizes the arrhythmias induced by NE during myocardial ischemia and reperfusion.
出处
《第四军医大学学报》
北大核心
2006年第9期784-787,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金面上项目(30370580)
全军医药卫生科研基金军队"十五"计划面上项目(01MB129)
