摘要
探讨持续性运动和耐力训练或注射丙酸睾酮对雄性大鼠体重增长、血脂水平以及肝脏、心脏及腓肠肌细胞内过氧化物体增殖活化受体α(PPARα)及其靶酶-酰基辅酶A氧化酶(AOX)的mRNA和PPARα蛋白量的影响。将40只SD大鼠随机分为4组:A为对照组,B为丙酸睾酮组,C为运动训练组,D为运动训练+丙酸睾酮组。丙酸睾酮给药方式:每天皮下注射5 mg/kg,每周6 d,共6周。运动方式:采用跑台,速度为25 m/min,每天1 h,每周6 d,共7周。结果发现:(1)B组和D组,实验后体重增长明显少于A组和C组(P<0.05);(2)D组的血清甘油三酯浓度,与A组相比,有降低的趋势(P>0.05),而明显低于C组(P<0.05)。总胆固醇、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇浓度,各组之间无明显差异;(3)各组间的PPARα和AOX的mRNA表达均无明显差异;(4)在肝脏和心脏,C组与D组的PPARα水平明显高于A组(均为P<0.001)。在腓肠肌,各组间PPARα水平无明显差异。结果表明:(1)注射丙酸睾酮和运动训练加注射丙酸睾酮可以明显控制体重的增长。这种作用可能与血清甘油三酯水平降低有关;(2)持续性运动在翻译及翻译后水平上调了心脏和肝脏PPARα基因的表达,对腓肠肌PPARα基因的表达无明显作用;(3)丙酸睾酮对心脏、肝脏和腓肠肌PPARα水平无明显调节作用,可能通过其他机制发挥作用。
The aim of study was to discuss the effects of testosterone propionate （TP） and/or endurance training on body weight, blood lipids, the expression of peroxisome proliferator-activated receptor α （PPARα） and acyl-CoA oxidase （AOX） in some tissues in rats. Forty adult male Sprague-Dawley rats were randomly divided into four groups： group A （control）, group B （TP）, group C （training） and group D （TP and training）. TP was administrated by subcutaneous injection at a daily dose of 5mg/kg body weight in group B and D for six weeks. Animals in group C and D received endurance training by running on the treadmill for one hour at a speed of 25m/min, six days per week, for seven weeks. The results showed that, first, the increased body weights in group B and D were less than in group A and C significantly （P〈0.05, respectively）. Second, serum triglyceride concentration in group D was lower than in group C significantly （P〈0.05）. Among the four groups there were no significant difference in serum concentration of total cholesterol, HDL-C and LDL-C. third, there were no significant differences in mRNA expression levels of PPARα and its target gene - acyl-CoA oxidase （AOX） in liver, heart and gastrocnemius muscle in all groups. Fourth, the level of PPARα protein was significantly higher in group C and D than that in control group （P〈0.001, respectively） in both liver and heart. No significant differences in PPARα protein expression were observed in all groups in gastrocnemius muscle.The results indicated that TP and TP combined with endurance training could reduce the weight gain. This effect might be related to the lower serum triglyceride concentration. The endurance training up-regulated the expression of PPARα gene in translation and post-translation level in liver and heart, but not in gastrocnemius muscle. The present findings demonstrated that endurance training but not TP could cause lipolysis possibly via PPARα.
出处
《天津体育学院学报》
CAS
CSSCI
北大核心
2006年第3期197-200,共4页
Journal of Tianjin University of Sport
基金
国家自然科学基金资助项目(项目编号:30150009)
关键词
过氧化物体增殖活化受体α
丙酸睾酮
训练
血脂
酰基辅酶A氧化酶
大鼠
peroxisome proliferator-activated receptor α （PPARα）
testosterone propionate （TP）
training
blood lipids
acyl-CoA oxidase （AOX）
rat
