摘要
目的探讨抑癌基因DPC4和PTEN蛋白在大肠癌的表达及临床意义。方法免疫组化SABC法和S-P法检测50例大肠癌组织中DPC4和PTEN蛋白的表达,以10例正常大肠组织作为对照。结果正常大肠组织DPC4和PTEN蛋白阳性表达率为100%。大肠癌组织DPC4蛋白在大多表达成(-)-(++),在癌组织表达程度显著低于正常组织DPC4蛋白表达程度。大肠癌组织PTEN蛋白阳性率分别为34%,与正常大肠组织PTEN表达的差异有显著性意义(P<0.01)。它们与细胞分化,临床分期及淋巴结转移有关。结论提示DPC4和PTEN蛋白表达与大肠癌密切相关,将来有可能作为大肠癌诊断和治疗过程中两种具有重要价值的生物学标记物。
Objective To explore the expression and clinical significance of PTEN and DPC4 gene in large intestine carcinoma. Methods Immunohistochemical staining （SABC - plus） and （SP - plus） was used to determine the expression of DPCA and PTEN in 50 cases of large intestine carcinoma. Results The total positive rate of DPCA and PTEN proteins was 100% repectively in non- tumor colorectal tissues irnmunohistochemistry revealed the degree of DPCA protein expression in normal tissues was much higher than in cancer tissues （ - ） - （ ＋ ＋ ） . The positive incidences of PTEN expression in large intestine carcinoma were 34 % and it had significant correlation to the difference of PTEN expression in normal large intestinetissue （P 〈 0.01） . The levels of DPCA and PTEN expression were correlated with size, clinical stage, malignancy of cancer and lymphnode metastasis, but not with gender, age and situation of tumor. Conclusions The expresion of DPCA and PTEN was closely correlated with the colorectal carcinoma, suggesting that DPCA and PTEN are potential biomarker for the Diagnosis and treatment of human colorectal carcinoma in the future.
出处
《锦州医学院学报》
2006年第1期1-4,共4页
Journal of Jinzhou Medical College
关键词
DPCA蛋白
PTEN蛋白
大肠癌
DPC4 gene protein
PTEN gene protein
large intestine carcinoma
