摘要
大豆DNA的提取是进行大豆分子生物学研究的基础。为了探索方便快捷的适合大豆分子生物学研究的DNA提取方法，在Aljanabi和Martinez 1997年提出的通用DNA快速盐提法的基础上，调整缓冲液中NaCl、Tris-HC1、EDTA浓度，提出一种适合大豆叶片DNA提取的改良盐提方法。该方法具有快速、简易和环境友好的特点。试验表明，该改良方法所提DNA凝胶条带清晰，无拖尾，浓度在1．5397～2．1039μg／L之间，0D260／OD280检测值在1．6913～1．8025之间，所提DNA适用于PCR，PCR结果理想。
In order to search a rapid, simple method extracting soybean DNA for molecular study, the concentration NaCl, Tris - HCl and EDTA were adjusted based on the method of extracting soybean DNA by Aljanabi and Martinez at 1997. An improved method of DNA salt extraction from soybean leaf was developed, which was rapid, simple and environment amiable. The experiments showed that the quality of DNA extracted by this improved method was much higher, DNA stripes were very clear without tagging, the concentration was 1. 5397 - 2. 1039μg/L and OD260/OD280 was 1. 6913 - 1. 8025.
出处
《河南农业科学》
CSCD
北大核心
2006年第5期35-38,共4页
Journal of Henan Agricultural Sciences
