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银杏叶提取物对实验性脊髓损伤后细胞凋亡的影响

ANTI-APOPTOSIS EFFECT OF GINKGO BILOBA EXTRACT ON RATS WITH EXPERIMENTAL SPINAL CORD INJURY
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摘要 目的观察银杏叶提取物（GBE）对脊髓损伤后神经细胞凋亡的影响.方法选择SD雄性大鼠（SPF级）30只,随机分成对照组和GBE组,均采用改良的Allen打击法制成中度急性脊髓损伤模型,术后GBE组给予GBE 0.75 mL/（kg·d）,对照组给予等量生理盐水.分别于术后1、3、5、7、14 d处死动物取材,采用流式细胞仪检测伤段脊髓细胞凋亡的情况.结果对照组和GBE组均发现凋亡细胞,均在5 d达到高峰,但GBE组凋亡细胞比率显著低于对照组（t=4.97～9.56,P〈0.05）.结论 GBE能够减少神经细胞的凋亡;抗凋亡可能是GBE缓解急性脊髓损伤的诸多机制之一. Objective To investigate the anti-apoptosis effect of Ginkgo biloba extract（GBE） after the spinal cord injury in SD rats, Methods Thirty SD male rats were randomly divided into two groups： control group and GBE group. The spinal cord injuries of the rats were induced by Allen＇s way by a 5 g×5 cm impact on the posterior T9 spinal cord in moderate degree. The rats in the control group were treated with normal saline, while those in the other group were administrated GBE 0.75 mL/ （kg · d）. The rats were sacrificed at 1, 3, 5, 7, 14 d after the injury. The cell apoptosis of the injured spinal cord was examined by flow cytometry. Results Positive expression of apoptotic cells appeared in both groups and reached their peak on third day after the injury. The cell apoptosis of the GBE group was significantly lower than that of the control group （t= 4.97- 9.56, P〈0. 05）. Conclusion GBE has neuroprotective effects on the experimentally injured spinal cord and can decrease the neuronal apoptosis.
作者 张忠礼 李鑫
机构地区 潍坊市人民医院创伤外科 潍坊医学院生理学教研室
出处 《齐鲁医学杂志》 2006年第2期136-137,共2页 Medical Journal of Qilu
基金 湖北省科技攻关课题资助项目(2003AA301C12)
关键词 银杏叶提取物 脊髓损伤 细胞凋亡 大鼠 Ginkgo biloba extract spinal cord injury apoptosis rats
分类号 R282.71 [医药卫生—中药学] R683.2 [医药卫生—骨科学]
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参考文献10

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齐鲁医学杂志
2006年 第2期

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