摘要
目的研究砷剂联合抗坏血酸协同诱导多发性骨髓瘤细胞株凋亡的机制。方法将培养的MM细胞株KM3分为:①对照组;②ATO单药组;③ATO+AA组;④AA单药组;⑤ATO+α生育酚组;⑥α生育酚单药组。分别检测细胞活力、细胞凋亡率、线粒体膜电位和细胞内GSH、H2O2水平。结果①ATO诱导MM细胞凋亡,AA增强其作用,α生育酚减弱其作用;②AA降低细胞内GSH水平,α生育酚升高细胞内GSH;③AA和α生育酚均升高细胞内H2O2水平;④ATO降低细胞线粒体膜电位,AA增强ATO的作用,α生育酚减弱其作用。结论砷剂诱导肿瘤细胞凋亡敏感性与细胞内GSH水平呈负相关,砷剂通过降低细胞线粒体膜电位,诱导细胞凋亡。AA通过降低细胞内的GSH水平,增加细胞对砷剂的敏感性。选择其他的还原剂无此作用。
Objective To study the underlying mechanism of combination of arsenic trioxide and ascorbic acid on the multiple myeloma cell strain KM3. Methods To divide the cultured cells into six groups： ①control group;②ATO alone ;③ATO plus AA ④AA alone ;⑤α-tocopherol alone ⑥（6-）ATO plus α-tocopherol, Cell viability was measured by Trypan blue dye, apoptosis rate and mitochondrial membrane potential was detected by FITC, the levels of GSH and H2O2 were measured by spectrophotometer. Results ①ATO can induce KM3 cell apoptosis, AA enhance its effect, while α-tocopherol decreased it. ②AA decreased intracellular GSH level, while α-toeopherol had the opposite function. ③Both AA and α- tocopherol can improve the H2O2 level in the cell. ④ATO can decrease cell＇s mitochondrial membrane pmential, AA would improve its function, and α-tocopherol would reduce this effect. Conclusion The sensitivity of cells to ATO related to the level of intracellular GSH. ATO induced KM3 cells to apoptosis through decreasing cellular mitochondrial membrane potential and causing the apoptotic cascade reaction. AA can decrease intracellular GSH level, and increased the sensitivity of cell to ATO. While other reduetants did not have this function.
出处
《国际输血及血液学杂志》
CAS
2006年第3期196-199,共4页
International Journal of Blood Transfusion and Hematology
