摘要
使用绿色荧光蛋白作为报告基因来研究目的蛋白的亚细胞定位得到广泛应用.使用稳定表达系统研究蛋白的亚细胞定位比较耗时,但可以先选择愈伤组织进行观察以确保构建的载体能够表达.优化了愈伤组织的培养条件,得到了质地疏松柔软的白色愈伤,不受叶绿体的荧光干扰,便于进行荧光观察.
GFP is widely used as a report gene to study protein subcellular localization. It is time-consuming to study protein subcellular location using stable expression system. However people could select callulas as meterials to verify whether the constructed vector could be expressed in a short time. The callulas culture condition was optimized. White and soft callulas were obtained. They are fitful to be observed under fluorescence microscope, having no interference of chloroplast.
出处
《河北师范大学学报（自然科学版）》
CAS
北大核心
2006年第3期343-345,共3页
Journal of Hebei Normal University：Natural Science
基金
国家转基因植物与产业化专项基金资助(JY03A14-01)
关键词
稳定表达
亚细胞定位
融合蛋白
愈伤组织
stable expression
subcellular localization
fusion protein
callulas
