摘要
根据GenBank收录的猪和牛附红细胞体16SrRNA基因序列设计1对通用引物,在其上游引物内侧又设计1条分别针对猪和牛附红细胞体的特异性引物。以这4条引物对出现附红细胞体病典型症状及疑似症状的猪和牛的血样DNA进行半套式-PCR扩增。结果显示,该反应阳性率为58.3%,低于临床解剖和镜检结果。对该基因的序列测定结果进行分析,表明不同动物附红细胞体的16SrRNA基因同源性在80%以上。从该基因来看,附红细胞体与立克次氏体没有同源性,而与肺炎支原体和穿透支原体亲缘关系较近。
A pair of primers was designed according to the 16S rRNA sequences of Eperythrozoon. Two single primer located downstream of the sense primer specific to the E.suis and wenyoni. Seminested PCR was subjected to the blood sample of bovine and swine. The positive rate is 58.3%, which is lower than the result of the general and microscope observation. The analysis of the DNA sequence shows high homology between every Eperythrozoons and with Ureaplasma gallorale and Mycoplasma penetrans. This is helpful to recognize the classification status and control of Eperythrozoon.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第3期262-264,共3页
Chinese Journal of Veterinary Science
基金
科技部科技攻关项目(2002BA518A16)
