摘要
目的通过研究不同碘摄入量的仔代大鼠脑组织抗氧化酶活力及其相关基因表达、过氧化产物含量,探讨碘对大鼠脑组织抗氧化能力的影响。方法将断乳后1个月的Wistar大鼠随机分为4组(即低碘组,适碘组,10倍碘组,50倍碘组),每组20只,分别饮用含不同碘浓度的水,每组每日总碘摄入量分别为<1、6.15、61.5和307.5μg/d。饲养3个月后随机交配,仔鼠饲养28d后,断头取其脑组织,称重,进行抗氧化酶活力及过氧化产物浓度测定,以RT-PCR方法检测抗氧化酶基因表达水平。结果与适碘组相比,大鼠脑内丙二醛(MDA)含量仅在低碘组明显升高(P<0.01),超氧化物歧化酶(SOD)活力在低碘组升高(P<0.05),在50倍碘组明显降低(P<0.01);SOD/MDA在低碘组和50倍碘组均明显降低(P<0.05,P<0.01);谷胱甘肽过氧化物酶(GSH-Px)活力在低碘组、10倍碘组和50倍碘组降低(P<0.05,P<0.01),GSH-Px/MDA也明显降低(P<0.01)。与适碘组相比,在10倍碘组和50倍碘组SODmRNA表达降低,各组GSH-PxmRNA表达无明显变化。结论碘缺乏和碘过量都可对仔代大鼠脑组织抗氧化能力有影响,造成脑组织自由基损伤,且低碘组比高碘组损伤明显;SOD和GSH-Px活力受转录、翻译、翻译后修饰等多水平调控。
Objective To evaluate the effects of iodine on antioxidative capability by observing activity of antioxidative enzymes and related gene expression, and peroxide content in the brain of rat offspring. Methods One-month weaning Wistar rats were divided into four groups（low iodine-H, normal iodine-NI, ten-fold high iodine-10HI and fifty-fold high iodine-50HI）, and fed with water containing different iodine concentration by adding potassium iodate respectively. Rats mate randomly after three months.The offspring were sacrificed at 28 days after birth, then the activity of SOD and GSH-Px, and MDA content in brain tissue were tested, and the SOD and GSH-Px mRNA expression were measured by RT-PCR. Results Compared with the NI group, only in LI group MDA content were increased significantly （P〈0.01）, but activity of SOD is higher in LI group（P〈0.05） and lower in 50HI group（P〈0.01）. SOD/MDA was decreased markedly（P〈0.05） in hoth LI group（P〈0.05） and in 50HI group; in LI, 10HI and 50HI groups, activity of GSH-Px is lower than that in NI group（P〈0.05,P〈0.01）, and GSH-Px/MDA shows the same tendency（P〈0.01）. SOD mRNA expression went down in IOHI and 50HI group, and GSH-Px mRNA expression showed no significant change. Conclusion Both iodine deficiency and iodine excess could lead to impairment of antioxidative capability in rat offspring brain, and iodine deficiency has more obvious impact. The activity of SOD and GSH-Px is regulated by many factors, including transcription, translation, post-translational modification, and so on.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第3期208-210,共3页
Journal of Environment and Health
基金
国家自然科学基金资助项目(30230330)
关键词
碘
脑
脂质过氧化
基因表达
Iodine
Brain
Lipid peroxidation
Gene expression
