摘要
目的:比较培养法、聚合酶链反应(PCR)和荧光定量PCR(FQ-PCR)在解脲支原体检测中的诊断价值和治愈判定价值。方法:对240例疑诊非淋菌性尿道炎(NGU)者,取尿道或宫颈内分泌物,同时进行培养、PCR和FQ-PCR法检测,并对三种方法均阳性的78例患者作治疗后的随访检测。结果:以2种以上(包括2种)检测方法检测的阳性结果为真阳性,2种检测结果均为阴性则定为真阴性。培养法的敏感性为78.57%(88/112),特异性为100%(128/128);PCR的敏感性为96.43%(108/112),特异性为93.75%(120/128);FQ-PCR的敏感性为100%(112/112),特异性为100%(128/128)。78例确诊患者经有效治疗后1周、2周、3周随访检测显示:培养法阴转快,1周的阴转率即为100%(78/78);PCR和FQ-PCR阴转均慢,2周后的阴转率分别为69.23%(54/78)和74.36%(58/78)。结论:培养法的特异性高,但敏感性低,必然产生假阴性;PCR敏感性高,但特异性稍差,易产生假阳性;FQ-PCR特异性和敏感性均好,适宜于推广运用。但在治愈判定中,培养法的价值更大,PCR和FQ-PCR的作用有限。
Objective： To compare the value of culture, polymerase chain reaction （PCR） and fluorescent-quantitative PCR （FQ-PCR） for diagnosis and evaluation of treatment on nongonococcal urethfitis （NGU） with Ureaplasma urealytium （UU）. Methods： 240 specimens from the genital tract of patients with equivocal NGU were detected by 3 methods, and 78 cases of patient with UU were followed-up and detected after having been treated with effective drugs. Results：If the results obtained by 2 methods appeared positive they were deemed as real positive, and if the results obtained by 2 methods appeared negative they were deemed as real negative. The sensitivity and specificity of culture were 78.57% （88/112） and 100% （128/128） respectively. That of PCR were 96.43% （108/112） and 93.75% （120/128）respectively. That of FQ-PCR were 100% （112/112） and 100% （128/128） respectively. After one,two or three weelss treatment 78 cases of the UU positive patients were fested by 3 methods and the results showed that the conyersion into negative of culture was quick, the conversion rate reached 100% （78/78） 1 week after treatment. However, that of PCR and FQ-PCR were slow, which only reached 69.23% （54/78） and 74.36% （58/78） 2 weeks after treatment espectively. Conclusion： The specificity of culture is high, but its sensitivity is low, which will certainly appear false negative; the sensitivity of PCR is high, but the specificity is low, which will easily appear false positive. However, both the sensitivity and specificity of FQ-PCR are high, which can be an effective method for diagnosis on NGU with UU. But in the evaluation of treatment, the value of culture is significantly higher than that of PCR and FQ-PCR.
出处
《泸州医学院学报》
2006年第2期121-123,共3页
Journal of Luzhou Medical College
关键词
解腺支原体
培养法
聚合酶链反应
荧光定量PCR
Ureaplasma urealytium
Culture
Polymerase chain reaction
Fluorescent-quantitaive PCR
