摘要
对分离鉴定的鸽源冠状病毒PSH株纤突蛋白S1基因进行RT-PCR法扩增、克隆和序列测定分析.结果表明其S1基因由1 626个核苷酸组成,编码541个氨基酸,S蛋白的切割识别位点为精氨酸-精氨酸-苯丙氨酸-精氨酸-精氨酸（RRFRR）,与常见的鸡传染性支气管炎病毒（IBV）S蛋白切割识别位点相似（RRF/SRR）.该病毒与火鸡蓝冠病病毒（TCV）Gh、G1株S1基因推导的氨基酸同源性仅为24.7%、25%,而与IBV H52、H120、M41、Beau、Conn、Gray、Hotel、SH1、SH2、SH5、SH6基因推导的氨基酸同源性在75.0%～99.6%,其中与SH2、SH5的氨基酸同源性更是达到了99.6%,进一步证明该冠状病毒可能来源于IBV.
The S1 gene of the pigeon original coronavirus （PSH） which was first isolated and identified in China was amplified by reverse transcription-polymerase chain reaction （RT-PCR） method, then it was cloned and sequenced. The results showed that the S1 gene of PSH was 1 626bp and encoded a polypeptide of 541 amino acids. The cleavage site sequence of S1 protein of PSH contains five consecutive basic amino acids, namely, Arg-Arg-Phe-Arg-Arg which is similar to the most IBV strains. Sequence data demonstrated that the complete S1 amino acid of PSH shared 75.0%-99. 6% similarity with the published S1 sequences of M41, H52, H120, Beau, Gray,Holte,Conn, SH1, SH2, SH5 and SH6 IBV strains. But it only shared 24. 7%,25% similarity with TCV Gh and G1 strain respectively. Based on these findings, the virus has been tentatively identified as a novel pathogenic field IBV strain, generated through recombination.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2006年第5期514-517,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
上海交大-昂立抗击"非典"科技基金(SARS03-03)
