摘要
卡那霉素(Km)对中嘉8号杨芽苗生长具有明显的抑制作用,随着浓度的提高,芽苗存活率逐渐降低,当Km≥60 mg/L时,存活率为0。Km对宽叶刺槐茎段的分化和芽的生长有较大影响,Km=10 mg/L时,促进分化,分化频率达100%,高出对照11.1%;Km≥40mg/L时,抑制芽的生长,芽的黄化率为100%;Km≥60mg/L时,没有芽的分化。因此,中嘉8号杨、宽叶刺槐遗传转化用卡那霉素为选择剂是合适的。
Kanamycin（Km） showed inhibition effects on the growth of Populus deltoids ＂63/69＂ with plant survival rates being reduced as concentration increesing. When Km≥ 60 mg/L, there was no plant survived. It also had inhibition effects on the regeneration and growth of shoots of Robinia pseudoacacia L. When Km = 10 mg/L, the shoots regenerated were promoted and shoots induction rates was 100 %, 11.1% over control. When Km ≥ 40 mg/L, shoots etiolation rates was 100 %. When Km = 60 mg/L, there was no shoots regenerated. Thus, Km is optimal for genetic engineering of the ＂63/69＂ and Robinia pseudoacacia L.
出处
《核农学报》
CAS
CSCD
北大核心
2006年第2期116-117,131,共3页
Journal of Nuclear Agricultural Sciences
基金
湖北省林业厅重点科技项目(编号:2002033)
关键词
杨树
刺槐
组织培养
抗生素
Populus deltoides
Robinia pseudoacacia L.
tissue culture
antibiotics
