摘要
AIM： To investigate the changes of lymphocyte subpopulations, especially CD4^＋CD25^ T regulatory cells in Smad3^-/- mice. METHODS： Hematological changes and changes of lymphocyte subpopulations were detected in Smad3＂/- mice using cell counter and flow cytometry, respectively, and compared to their littermate controls. RESULTS： The numbers of neutrophils and lymphocytes in peripheral blood were significantly increased in Smad3^-/- mice compared to littermate controls. CD19^＋ expressing cells in blood and spleen, and CD8^＋ T cells in thymus were all markedly decreased in Smad3^-/- mice. More important, Smad3^-/- mice had an increased population of CD4^＋CD25^＋ T cells in peripheral lymphoid tissues, including thymus, spleen, and lymph nodes. CONCLUSION： These observations suggest that the changes of lymphocyte subpopulations might play a role in susceptibility to inflammation of Smad3^-/- mice.
瞄准：调查淋巴细胞潜水艇人口的变化，在 Smad3 的特别 CD4 (+) CD25 (+) T 规章的房间(-/-) 老鼠。方法：淋巴细胞潜水艇人口的 Hematological 变化和变化在 Smad3 被检测(分别地， -/-) 鼠标使用房间柜台和流动血细胞计数控制。结果：在外部血的 neutrophils 和淋巴细胞的数字显著地在 Smad3 被增加(与同窝出生的人控制相比的 -/-) 老鼠。在血和怒气表示房间的 CD19 (+) ，和在胸腺的 CD8 (+) T 房间都显著地在 Smad3 被减少(-/-) 老鼠。更重要， Smad3 (-/-) 老鼠在外部淋巴组织，包括的胸腺，怒气，和淋巴有 CD4 (+) CD25 (+) T 房间的一张增加的人口节点。结论：这些观察建议淋巴细胞潜水艇人口的变化可能在危险性起一个作用到 Smad3 的发炎(-/-) 老鼠。
