摘要
采用苯胺蓝脱色法,从活性污泥中分离筛选得到2株具有分泌木质素降解过氧化物酶能力的细菌菌株PKE117和PKE225.根据生理生化反应结果和16SrDNA序列分析,初步确定菌株PKE117为Pseudomanas属的1个新种,PKE225为Pseudomanas thermaerum的1个新菌株.对于菌株PKE117的发酵液依次经DEAE-纤维素32、Sephadex G-75凝胶过滤纯化,木素过氧化物酶的藜芦醇氧化比活力从0.87U/mg提高到204.5U/mg,酶的纯化倍数为235.1,回收率15%.
Two strains that could use lignin as role carbon source and excrete peroxidases were isolated from activated sludge. Both strains are belonged to Pseudomanas based on morphological, physio-biochenical characterizatics and homology identification of 16S rDNAsequence, in which strain PKE117 is identified as a new species, while the strain PKE225 is identified as Pseudomanas thermaerum. Crude enzyme in liquid fermentation of PKEll7 was analyzed with DEAE-cellulose 32 ion-exchanger resin chromatography and Sephadex G-75 gel filtration in proper order. The lignin peroxidase specific activity increases from 0.87U/mg to 204.SU/mg, purification multiple is 235.1 and callback rate is 15%.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2006年第5期981-985,共5页
Environmental Science
基金
中国博士后科学基金项目(2005037279)
关键词
木质素
分离鉴定
木素过氧化物酶
纯化
lignin
imlation and identification
lignin peroxidase
purification
