摘要
研究了菌株DN2降解烟碱的特性和对烟草废弃物中烟碱的降解情况。结果表明,该菌降解烟碱的最适条件为接种量5%,温度30℃,初始pH值6.5。在该条件下,对初始烟碱浓度为500mg/L的降解过程进行考察。结果表明,未经烟碱诱导的降解曲线呈倒S曲线,半衰期为17.43h;经烟碱诱导的降解曲线符合Eckenfelder动力学模型,半衰期为4.10h。添加0.1%(质量分数)葡萄糖,可提高菌株DN2的烟碱耐受浓度,达5000mg/L。菌株DN2能够降解烟草废弃物水提液中的烟碱(烟碱含量约为2220mg/L),60h时烟碱的降解率为95.22%,表明该菌在治理烟碱污染环境方面具有应用价值。
The kinetics of nicotine degradation by 0. intermedium DN2 and its application in tobacco waste were investigated. The results showed that the optimum temperature of nicotine degradation by O. intermedium was 30℃, the pH value was 6.5 and a mount of inoculum was 5 %. Under above conditions, the kinetics of nicotine degradation of initial concentration 500 mg/L was studied. The results indicated that the degradation process of nicotine with no-induced strain DN2 followed inverse S-shaped curve, and degradation process of nicotine with induced cells of DN2 followed Eckenfelder mode. The half life of nicotine degradation was 17.43 h and 4.10 h, respectively. And the results also showed that tolerance of 0. intermedium DN2 to nicotine was up to 5000 mg/L when 0. 1% about 95.22 % by strain nicotine pollution. of glucose was added. DN2 in 60 h incubation Nicotine （2 220 rag/L） in extract of tobacco wastes degraded , indicating that strain DN2 was of application value in treatment nicotine pollution.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2006年第3期47-50,88,共5页
China Biotechnology
