摘要
目的:探讨6种常用抗肿瘤药物长春新碱、阿霉素、平阳霉素、顺铂、5-Fu、环磷酰胺与维拉帕米联合对KBV200细胞P-糖蛋白表达的影响。方法:以喉癌KBV200细胞株为研究对象,通过流式细胞术检测6种抗肿瘤药物作用KBV200细胞的P-糖蛋白表达以及联合维拉帕米对P-糖蛋白表达的影响。结果:维拉帕米分别与长春新碱、阿霉素、平阳霉素、环磷酰胺联合作用,使KBV200细胞的P-糖蛋白表达下降80.17%、89.69%、19.19%及53.48%。其中维拉帕米与长春新碱、阿霉素作用较强。结论:维拉帕米分别与长春新碱、阿霉素、平阳霉素、环磷酰胺联合作用后,P-糖蛋白的表达水平明显下降,对KBV200细胞耐药性的逆转有一定作用。
Objective： To observe the effect of verapamil and 6 chemotherapeutic agents vincristine,adriamycin,bleomycin,cyclophosphamide,5-Fu,cisplatin on the P-glucoprotein （P-gp） of the KBV200 cell line. Methods： Flow cytometer（FCM） assay was used to investigate the expression of P-gp on the KBV200 cell line in the different conditions. Results： Verapamil act with vincristine, adriamycin,pingyangmycin and cyclophosphamide separately decreased expression of P-gp on KBV200 cell line by 80.17%,89.69%,19.19% and 53.48% Among all the combinations, the synergistic effects of varapamil act with vincristine and adriamycine show a better effect. Conclu-sion： Verapamil act with vincristine, adriamycin, pingyangrnycin and cyclophosphamide separately can decrease expression of P-gp on KBV200 cell line significantly, and can reverse the drug resistance of KBV200 cell line.
出处
《重庆医科大学学报》
CAS
CSCD
2006年第3期369-372,共4页
Journal of Chongqing Medical University
关键词
KBV200细胞株
多药耐药
耐药逆转
P糖蛋白
维拉帕米
KBV200 multidrug resistance cell line
Multidrug resistance
Reversion of multidrug resistance
P-gp
Verapamil
