摘要
目的:探讨卵巢癌凋亡抑制基因Survivin的表达、细胞凋亡关系及临床意义。方法:用末端脱氧核糖核酸转移酶介导的TUNEL法和免疫组织化学技术检测50例卵巢癌组织原位凋亡(AI)、Survivin的表达。结果:50例卵巢癌组织中Survivin阳性表达率72%(36/50),其表达与AI相关。Survivin阴性表达组的5年生存率显著高于阳性表达组(P<0.05);Cox模型多因素分析表明,高AI(≥1.7%)患者累积生存率较高(P<0.05)。结论:凋亡抑制基因Survivin的异常表达与卵巢癌细胞凋亡抑制及不良预后密切有关。
Objective： To investigate clinical significance of survivin expression, cellular apoptosis in ovarian cancer. Method： The apoptosis in sit ovarian cancer were identified by the terminal deoxynucleotidy transfer mediated dUTP-biotin nick end labeling （TUNEL） and survivin expression was detected by immunohistologic staining in 50 cases of ovarian cancer. Result： Survivin was expressed in 36 of 50 ovarian cancer （72%）. The correlation was found between survivin expression, cellular apoptosis. The 5 year survival rate in the group of survivin-negative cases was significant higher than in the group of survivin-positive cases （P 〈 0.05）. In Cox regression analysis, survivin expression and cellular apoptosis index （AI） were indpendent risk factors for prognosis. Conclusion： Survivin, a inhibitor gene of apoptosis, expression abnormal correlated with apoptosis inhibition, poor prognosis in ovarian cancer.
出处
《重庆医科大学学报》
CAS
CSCD
2006年第3期388-390,共3页
Journal of Chongqing Medical University
