摘要
目的:用高效液相色谱法测定金银花中木犀草素的含量。方法:采用ShimadzuC18柱(150mm×4.6mm,5μm),流动相为甲醇-0.2%H3PO4溶液(50:50),流速1.0ml/min,检测波长254nm,柱温室温,用外标法定量,测定金银花中木犀草素的含量。结果:木犀草素的线性范围0.0325～0.39μg,r=0.9999,回收率99.65%,RSD1.24%。结论:该方法简便,快速,线性关系良好,可作为金银花的含量测定。
Objective： The purpose is to determine the content of luteolin from the flowers of Lonicera japonica. Methods： The HPLC method was used to determine the content of luteolin from flowers of Lonicera japonica. The analysis was carried out on Shimadzu C18 colunm（150mm × 4.6mm, 5 μm）.The mobile phase was MeOH-0.2% H3PO4 （50：50）, flow-rate 1.0ml/min and wave-length 254nm. The column temperature was room temperature. Results： The method is simple and of a good linear relationship.The liner range of lnteolin is 0.0325 -0.39μg and gives a correlation （r） of 0.9999. The recovery is 99.65 % and RSD 1.24%. Conclusion： The method is easy to determine the content of luteolin from the flowers of Lonicerajaponica.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第5期221-222,共2页
Food Science
