摘要
目的检测雄黄诱导白血病细胞凋亡能力,探讨分子机制。方法应用形态学观察细胞凋亡,计算细胞凋亡率,免疫组化方法检测白血病细胞bcl-2蛋白表达。结果雄黄可诱导白血病细胞发生典型的凋亡,同时雄黄可使白血病细胞bcl-2蛋白表达呈时间依赖性下降。结论雄黄通过影响白血病细胞bcl-2蛋白的表达,从而导致白血病细胞发生凋亡,这是其治疗白血病的重要分子机制。
Objective To investigate the apoptosis of leukcmia ceils induced by realgar,and illustrate tile possible molecular mechanisms. Methods Apoptosis was detected by morphological observation,and bel-2 expression by immunohistochemieal staining with specific antibodies. Results It found that apoptosis was induced in leukemia cells and bcl-2 expression down-regulaled by realgar. Conclusions Down-regulation of bel 2 expression appears to play an iniportant role in apoptosis of the differentiated leukemia cells induced by realgar.
出处
《齐齐哈尔医学院学报》
2006年第4期396-397,共2页
Journal of Qiqihar Medical University
