期刊文献+

除莠霉素A结构类似物M3、M4的分离鉴定及对肿瘤细胞的活性 被引量:6

Purification,structure elucidation and antitumor activity of minor antibiotics M3 and M4 in the fermentation broth of Streptomyces hygrosopicus NND-52
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摘要 吸水链霉菌NND-52发酵液的上清液经乙酸乙酯萃取,硅胶柱粗分离,经制备型高效液相色谱仪分离纯化,得除莠霉素A(herbimycinA)、黄色M3纯品和浅黄色M4粗品,粗品结晶后获得M4的白色针状结晶;通过基本理化性质和光谱分析,确定M3、M4分别为除莠霉素C(herbimycinC)、双氢除莠霉素A;噻唑蓝(MTT)法检测除莠霉素A、除莠霉素C及双氢除莠霉素A对Vero、B16-F10细胞增殖的影响,对肿瘤细胞活性强度顺序为:除莠霉素C>除莠霉素A>双氢除莠霉素A。除莠霉素C可能是一种更具潜力的抗肿瘤抗生素。 Antibiotic M3 and M4 were isolated from the cultured broth of Streptomyces hygroscopicus NND-52 by ethyl acetate extraction, silica gel column chromatography and preparative high performance liquid chromatography. Basic physical and chemical characteristics and spectra data showed antibiotic M3 and M4 are herbimycin C and dihydroherbimycin A, respectively. The effects of herbimycin A, herbimycin C and dihydroherbimycin A on proliferation of Vero and B16-F10 cells were investigated by MTT assay. Results showed that their antitumor activities were as follows: herbimycin C〉herbimycin A〉dihydroherbimycin A. Compared with herbimycin A, herbimycin C is a more potentially applicable antitumor antibiotic.
出处 《中国抗生素杂志》 CAS CSCD 北大核心 2006年第5期274-277,共4页 Chinese Journal of Antibiotics
关键词 分离 结构 除莠霉素C 双氢除莠霉素A 抗肿瘤 Isolation Structure Herbimycin C Dihydroherbimycin A Antitumor
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参考文献12

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