摘要
目的探讨MTT法体外药敏试验在复发转移性乳腺癌化疗中的价值。方法取35例复发转移性乳腺癌患者肿瘤组织,简易机械法制备细胞悬液,应用MTT法体外药敏试验测定其对10种常用化疗药物的敏感性,并指导临床用药,同时与临床疗效进行相关分析。结果MTT法检测复发转移乳腺癌患者药敏试验的成功率为91.4%,体外药敏试验的敏感性为86.4%,特异性为80.0%,阳性预测值为90.5%,阴性预测值为72.7%,总的预测准确率为84.4%。结论MTT法可作为临床上指导复发转移乳腺癌个体化化疗的有效方法。
Objective To explore the value of Drug Sensitivity by MTT assay in vitro for patients with breast cancer. Methods 35 breast tumor samples were analyzed by MTT chemosensitivity assay with 10 anticancer drugs. Thecoselation between chcmosensitivity and clinical response was analyzed. Results MTT assay was Successful in 91.4% （32/35） with a sensitivity of 86.4% and a specificity of 80.0%.The positive predictive value was 90.5% an d the negative predictive value Was 72.7% in the study.The overall coincident rate Was 84.4%. Conclusion MTT assay in vitro is an eficient and reliable method for the chemosensitivity assessment of bumont breast cancer in clinic.
出处
《中国实验诊断学》
2006年第5期471-472,共2页
Chinese Journal of Laboratory Diagnosis
