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抑制survivin表达对前列腺癌细胞pc-3凋亡的影响 被引量：1

Effect of inhibition of survivin expression on the apoptosis of prostate cancer cell pc-3
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摘要 [目的]研究survivin与前列腺癌的关系,探讨survivin对前列腺癌细胞pc-3凋亡的影响及其机制。 [方法]运用RNA干扰技术阻断survivin在前列腺癌细胞pc-3中的表达,采用流式细胞计数等方法检测survivin 被阻断后,对前列腺癌细胞pc-3凋亡的影响。[结果]阻断survivin在前列腺癌细胞pc-3中的表达后,能够引起 pc-3细胞的凋亡,其机制可能是由于活化了凋亡通路的caspase-3。[结论]Survivin可以做为治疗前列腺癌的一个潜在的基因靶点。 [Objective] To evaluate the relationship between survivin and prostate cancer and the effect of survivin on the apoptosis of prostate cancer and it＇s probable mechanism. [Methods] Survivin expression on prostate cancer cell pc-3 was interrupted by RNA interference （RNAi） technigae and the apoptosis ratio of prostate cancer cell PC-3 was determined by flow cytometer. [Results] Interruption of the survivin expression of prostate cancer cell pc-3 could induce the apoptosis of pe-3 cells. The mechanism might be that the apoptosis pathway of caspase-3 was activated. [Conclusion] Survivin might be act as a potential therapeutic target for prostate cancer.
作者 黄谷 张明 王海坤
机构地区 武警广东总队医院外三科
出处 《武警医学院学报》 CAS 2006年第1期14-16,共3页 Acta Academiae Medicinae CPAPF
关键词 RNA干扰 SURVIVIN PC-3细胞 凋亡 CASPASE-3 RNA interference Survivin pc-3 cell Apoptosis Caspase-3
分类号 Q255 [生物学—细胞生物学] R979.1 [医药卫生—药品]
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参考文献11

  • 1Ambrosini G,Adida C,Altieri DC,et al.A novel anti-apoptosis gene,survivin,expressed in cancer and lymphoma[J].Nat Med,1997,3 (8):917-921.
  • 2陈忠斌,于乐成,王升启.RNA干扰作用(RNAi)研究进展[J].中国生物化学与分子生物学报,2002,18(5):525-528. 被引量：35
  • 3Fire A,Xu S,Montgomery MK,et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J].Nature,1998,391 (6669):806-811.
  • 4Jorgensen RA,Cluster PD,English J,et al.Chalcone synthase cosuppression phenotypes in petunia flowers:comparison of sence vs antisense constructs and single-copy vs.complex T-DNA sequences[J].Plant Mol Biol,1996,31 (5):957-973.
  • 5Cogoni C,Irelan J T,Schumacher M,et al.Transgene silencing of the al 1 gene in vegetative cells of neureospora is mediated by a cytoplasmic effector and does not depend on DNA-DNA interacttionsor DNA methylation[J].EMBO J,1996,15 (12):3153-3163.
  • 6Adida C,Crotty PL,Mc Grath J,et al.Developmentally regulated expression of the novel cancer anti2apoptosis gene survivin in human and mouse differentiation[J].Am J Pathol,1998,152 (1):43.
  • 7Ambrosini G,Adida C,Altieri DC,et al.A novel anti2apoptosis gene,survivin,expressed in cancer and lymphoma[J].Nat Med,1997,3 (8):917.
  • 8Tamm I,Wang Y,Sansville E,et al.IAP2 family protein surviving inhibits caspase activity and apoptosis induced by Fas (CD95),Bax,caspases,and anticancer drugs[J].Cancer Res,1998,58(23):5315.
  • 9Sarela AI,Macadam RC,Farmery SM,et al.Expression of the anti-apoptosis gene,survivin,predicts death from recurrent colorectal carcinoma[J].Gut,2000,46 (5):645.
  • 10Monzo M,Rosell R,Felip E,et al.A novel anti2apoptosis gene:Re2expression of survivin messenger RNA as a prognosis marker in non2 small2celllung cancer[J].J Clin Oncol,1999,17 (7):2100.

二级参考文献23

  • 1[1]Fire A, Xu S, Montgomery M K, Kostas S A, Driver S E, Mello C C: Potent and specific genetic interference by double-strand RNA in Coenorhabditis elegans. Nature, 1998,391(6669) :744 ～ 745
  • 2[2]Hunter C P. Gene silencing: shrinking the black box of RNAi. Curr Biol, 2000, 10(4):R137～ 140
  • 3[3]Zamore P D, Tuschl T, Sharp P A, Bartel D P. RNAi: double-strand RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell, 2000,101: 25 ～ 33
  • 4[4]Caplen N J, Panish S, Imani F,Fire A,Morgan R A. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc Natl Acad Sci USA ,2001 ,98(17) :9742 ～9747
  • 5[5]Nykanen A, Haley B, Zamore P D. ATP requirements and small interferings RNA structure in the RNA interference pathway. Cell, 2001,107(3) :309 ～ 3021
  • 6[6]Matzke M,Matzke A J,Kooter J M. RNA:guiding gene silencing. Science ,2001,293(5532): 1080 ～ 1083
  • 7[7]Lipardi C, Wei Q, paterson B M. RNAi as random degradative PCR.siRNA primers convert mRNA into dsRNAs that are degraded to generate new siRNAs. Cell, 2001, 107(3):297～307
  • 8[8]Sijen T, Fleenor J, Simmer F, Thijssen K L, Parrish S, Timmons L,Plasterk R H, Fire A. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell, 2001, 107(4) :465 ～ 476
  • 9[9]Nishikura K. A short primer on RNAi: RNA-directed RNA polymerase acts as a key catalyst. Cell, 2001, 107(4) :415 ～ 418
  • 10[10]Boutla A, Delidakis C, Livadaras l,Tsagris M,Tabler M. Short 5'-phosphorylated double-stranded RNAs induce RNA interference in Drosophila. Curr Biol, 2001, 11(22): 1776 ～ 1780

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武警医学院学报
2006年 第1期

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