摘要
目的研究类(RA)风湿性关节炎患者外周血CD4+CD25+、CD4+、CD25+淋巴细胞亚群的变化及意义,探索其在类风湿性关节炎发病中的作用。方法采用二色荧光抗体标记法,以流式细胞仪对22例RA患者和18例正常人外周血CD4+CD25+、CD4+、CD25+淋巴细胞亚群等进行检测,以CD25抗原荧光强度10的细胞定义为CD 25 h igh细胞,分析其百分率和荧光强度,并结合临床资料进行相关分析。结果RA患者外周血CD4+CD25+细胞、CD4+CD25h igh细胞和CD25+细胞占总淋巴细胞的百分率与正常对照组无显著差异(分别为10.33±4.41 vs 8.92±3.21,0.57±0.39 vs0.44±0.22,14.17±5.05 vs 13.91±3.38,P>0.05),而CD4+细胞数较正常增高(39.13±3.77 vs 33.83±7.22,P<0.05);活动组RA患者外周血CD4+、CD25+荧光抗原强度高于正常对照组(7.69±3.44 vs 5.20±0.91和3.27±0.77vs 2.61±0.24,P<0.05),亦高于稳定组患者(P<0.05)。结论外周血CD4+CD25+调节性T细胞对类风湿性关节炎发病和病情判断等无重要意义,而CD4+T细胞在类风湿性关节炎发病中可能起一定作用。
Objective To investigate the expression and significance of peripheral blood CD4^＋, CD25^＋ and CD4^＋ CD25^＋ cellls in patients with rheumatoid arthritis（RA） ,and their roles in the pathogenesis of RA. Methods The percentage and fluorescence intensities of peripheral blood CD4^＋, CD25^＋ and CD4^＋ CD25^＋ subpopulations from 22 RA and 18 normal controls were measured with flow cytometry, Labolatory data were analyzed in combination with clinical data. The CD25^＋ cells whose fluorescence intensities were equal to or more than 10 were defined as the CD25high cells. Results There was no significant difference in the percentage of peripheral blood CD4^＋CD25^＋ , CD4^＋CD25high and CD25^＋ cells between RA patients and normal control group （ 10.33% ± 4.41% vs 8.92% ± 3.21% , 0.57% ± 0.39% vs 0.44% ± 0.22% and 14. 17% ± 5.05% vs 13.91% ± 3.38 %, respectively, P 〉0.05 ）, however CD4^＋ T cells were higher in RA patients than that in normal control group （39.13% ± 3.77% vs 33.83%± 7.22%, P 〈 0.05 ）. Fluorescence intenaities of CD4^＋ and CD25^＋ in active RA patients were higher as compared with non - active RA patients and normal control group （ 7.69 % ± 3.44 % vs 5.20 % ± 0.91%, 3.27 % ± 0.77 % vs 2.61% ± 0. 24 %, P 〈 0.05 ）. Conclusion Peripheral blood CD4^＋ CD25^＋regulatm7 T cells is not important in the pathogenesis and severity judgement of RA, while CD4^＋ cells may play a role in the pathogenesis of RA.
出处
《临床和实验医学杂志》
2006年第5期445-447,共3页
Journal of Clinical and Experimental Medicine
