期刊文献+

呼吸机所致肺损伤家兔HSP70和NF-κB表达的意义 被引量:1

Expression of heat shock protein 70 and nuclear factor-κB in ventilator-induced lung injury in rabbit
下载PDF
导出
摘要 目的研究呼吸机所致肺损伤(VILI)炎症反应中热休克蛋白70(HSP70)和核因子-κB(NF-κB)表达的意义,以及加用呼气末正压(PEEP)的影响。方法健康成年新西兰白兔30只,实施麻醉和气管切开术后分别接受不同潮气量(VT)的通气(通气时间均为4h)。随机分为以下三组PEEP组(VT=40ml/kg,PEEP=3cmH2O,n=12);ZEEP组(VT=40ml/kg,PEEP=0cmH2O,n=12);对照组(VT=10ml/kg,n=6),始终以正常条件通气。机械通气开始后4h经颈动脉放血处死动物。在光镜下观察肺组织的病理学改变,Westernblot检测家兔肺组织HSP70及NF-κB的表达。结果家兔在致伤机械通气4h后,光镜下可见肺组织有不同程度的损伤;各组肺组织HSP70、NF-κB表达均显著增加,且ZEEP组二者呈负相关。与ZEEP组比较,PEEP组肺组织的病理损伤明显减轻,NF-κB表达明显减少。结论HSP70可通过抑制NF-κB的活性,保护肺组织避免VILI;在机械通气过程中加用PEEP也可通过减少NF-κB的表达减轻肺组织损伤。 Objective To investigate heat shock protein 70 (HSP70) and nuclear factor-kappa B( NF-kB) expression in the lung after mechanical ventilation with PEEP(positive end expiratory pressure)in a rabbit model of ventilator-indueed lung injury (VILI). Methods Thirty healthy adult white rabbits were randomly divided into three groups. After anesthesia and tracheostomy were installed, rabbits received ventilation with different tidal volumes for 4 hrs. In PEEP group, rabbits received ventilation withVT = 40ml/kg, PEEP = 3emH2O; group, rabbits received ventilation with VT=40ml/kg, PEEP=0cmH2O; and NVC group, with normal ventilation with VT= 10ml/kg. The different modalities of ventilation were maintained for 4 hours. After 4 hours, rabbits were killed by exsanguination, Lung histopathology was examined and compared among three groups. Expression of HSP70 and NF-kB were determined by using Western-blot technique. Results There were marked pathological changes in the lung tissue in group ZEEP, but pathological changes were relatively milder in group PEEP. After injurious ventilation for 4 hours, there was a significantly higher expression of HSP70 and NF-kB in group ZEEP, while that of NF-kB was negatively correlated with HSP70 levels. There was a significant lower expression of NF-kB in the lung of group PEEP,Conclusion We postulate that HSP70 expression protects the lung against ventilator-induced lung injury by down-regulating expression of NF-kB in the lung. Mechanical ventilation with PEEP can protect the lung against ventilator-induced lung injury through down-regulation of NF-kB in the lung.
出处 《解放军医学杂志》 CAS CSCD 北大核心 2006年第5期442-443,共2页 Medical Journal of Chinese People's Liberation Army
基金 全军"十五"医药卫生科研基金资助项目(01MA105)
关键词 呼吸窘迫综合征 成人 热休克蛋白质70 NF-KB respiratory distress syndrome, adult heat-shock proteins 70 NF-kappa B
  • 相关文献

参考文献5

  • 1Dreyfuss D,Saumon G.Ventilator-induced lung injury:lessons from experimental studies.Am J Respir Crit Care Med,1998,157:294.
  • 2Didier D,Jean DR,Georges S.On the physiologic and clinical relevance of lung-borne cytokines during ventilator-induced lung injury.Am J Respir Crit Care Med,2003,167:1467.
  • 3Slutsky AS.Lung injury caused by mechanical ventilation.Chest,1999,116:9s.
  • 4Vreugdenhil HA,Haitsma JJ,Jansen KJ et al.Ventilator-induced heat shock protein 70 and cytokine mRNA expression in a model of lipopolysaccharide-induced lung inflammation.Intensive Care Med,2003,29:915.
  • 5邱海波,杨毅,周韶霞,许红阳,刘少华,郑瑞强,黄英姿.核因子-κB在小鼠急性肺损伤中的作用[J].中华急诊医学杂志,2003,12(1):15-17. 被引量:26

二级参考文献1

共引文献25

同被引文献10

  • 1张修礼,曲建慧,孙自勤,李兆申.低浓度乙醇诱导与紫外线照射对HepG2细胞周期及p53/p21蛋白表达的影响[J].解放军医学杂志,2006,31(12):1166-1168. 被引量:1
  • 2Girard PM, Pozzebon M, Delacote F, et al. Inhibition of S-phase progression triggered by UVA-induced ROS does not require a functional DNA damage checkpoint response in mammalian cells [J]. DNA Repair (Amst), 2008, 7(9): 1500-16.
  • 3Branzei D, Foiani M. Regulation of DNA repair throughout the cell cycle[J]. Nat Rev Mol Cell Biol, 2008, 9(4): 297-308.
  • 4Naugler WE and Karin M, NF-kappaB and cancer-identifying targets and mechanisms [J]. Curr Opin Genet Dev, 2008, 18(1): 19-26.
  • 5Edwards MR, Bartlett NW, Clarke D, et al. Targeting the NF- kappaB pathway in asthma and chronic obstructive pulmonary disease[J]. Pharmacol Ther, 2009, 121(1): 1-13.
  • 6Gagliardo R, Chanez P, Mathieu M, et al. Persistent activation of nuclear factor-kappaB signaling pathway in severe uncontrolled asthma[J]. Am J Respir Crit Care Med, 2003, 168(10): 1190-8.
  • 7Campbell KJ, Rocha S, Perkins ND. Active repression of antiapoptotic gene expression by RelA(p65) NF-kappa B [J]. Mol Cell, 2004, 13(6): 853-65.
  • 8Hellings PW, Prokopakis EP. Global airway disease beyond allergy [R]. Curt Allergy Asthma Rep, 2010, 10(2): 143-9.
  • 9Merendino AM, Bucchieri F, Gagliardo R, et al. CD40 ligation protects bronchial epithelium against oxidant-induced caspase- independent cell death [J]. Am J Respir Cell Mol Biol, 2006, 35 (2): 155-64.
  • 10郭玲,何英杰,郑莉,吕超,丁振华.荧光定量分析miR-376b在UVB照射的人角质细胞中的差异表达[J].解放军医学杂志,2010,35(8):1039-1039. 被引量:1

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部