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RNA干涉法观察成纤维细胞生长因子18对成牙本质样细胞涎磷蛋白表达的影响 被引量：2

Investigation of the effect of FGF18 on DSPP expression of MDPC-23 by RNA interference
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摘要 目的:通过RNA干涉的方法研究成纤维细胞生长因子18(Fibroblast Growth Factor18,FGF18)对成牙本质样细胞中牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)表达的影响。方法:RNA干涉质粒pH1-siFGF18瞬时转染成牙本质样细胞MDPC-23,RT-PCR检测FGF18和DSPP的表达,观察FGF18低表达对DSPP表达的影响。结果:瞬时转染RNA干涉质粒后,MDPC-23细胞内FGF18表达明显减少,与此同时DSPP的表达量也明显减少。结论:FGF18可以调控DSPP的表达,影响成牙本质细胞的分化。 AIM ：To investigate the effect of FGFI8 on DSPP expression of MDPC -23 by means of RNA interference. METHODS： MDPC- 23 cells were transiently transfected with RNA interference plasmid pHI/siFGF18, then the expression of FGF18 and DSPP were examined by RT - PCR. RESULT： With the decrease of FGF18 expression by RNA interference, the DSPP expression also decreased. CONCLUSION： FGF18 may modulate the expression of DSPP and participate in the development and differentiation of odontoblast.
作者 姜永 肖明振 杨帆 何文喜 范晓敏
机构地区 第四军医大学口腔医学院 第四军医大学口腔医学院牙体病科
出处 《牙体牙髓牙周病学杂志》 CAS 2006年第5期243-245,共3页 Chinese Journal of Conservative Dentistry
基金 国家自然科学基金资助项目(30300385)
关键词 成纤维细胞生长因子18 牙本质涎磷蛋白 MDPC-23 RNA干涉 瞬时转染 fibroblast growth factorl8 dentin sialophosphoprotein MDPC - 23 RNA interference transient transfection
分类号 R780.2 [医药卫生—口腔医学]
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参考文献10

  • 1Lesot H, Lisi S, Peterkova R,et al. Epigenetic signals during odontoblast differentiation[J]. Adv Dent Res, 2001, 15 (8) :8 - 13
  • 2Kettunen P, Laurikkala J, Itaranta P, et al. Associations of FGF -3 and FGF - IO with signaling networks regulating tooth morphogenesis[J]. Dev Dyn, 2000, 219(3) :322 -332
  • 3Usui H, Shibayama M, Ohbayashi N, et al. Fgfl8 is required for embryonic, lung alveolar development [J]. Biochem Biophys Res Commun, 2004,322 ( 3 ) : 887 - 92
  • 4Reinhold MI, Abe M, Kapadia RM, et al. FGFI8 represses noggin expression and is induced by calcineurin[J]. J Biol Chem, 2004,279(37) :38209 -38219
  • 5杨帆,肖明振,赵守亮.小鼠牙胚fgf18的基因克隆和序列分析[J].临床口腔医学杂志,2003,19(4):200-202. 被引量：5
  • 6M.K. Hajihosseini, J.K. Heath. Expression patterns of fibroblast growth factors - 18 and -20 in mouse embryos is suggestive of novel roles in calvarial and limb development[J]. Mechanisms of Development, 2002,113( 1 ) :79 -83
  • 7Hanks CT, Fang D, Sun Z, et al. Dentin - specific proteins in MDPC23 cell line[J]. Eur J Oral Sci, 1998, 106( Suppl 1 ) :260- 266
  • 8Downward J. RNA interference [ J ]. BMJ, 2004,328 ( 7450 ) :1245 - 1248
  • 9Shimokawa T, Furukawa Y, Sakai M, et al. Involvement of the FGFI8 gene in colorectal carcinogenesis, as a noveld downstream target of the - catenin/T - cell factor complex [ J ]. Cancer Research, 2003, 63(19): 6116-6120
  • 10Takashi SH,Toru OG, Akiko YO, et al. Regulation of osteoblast chondrocyte and osteoblast functions by fibroblast growth factors (FGF) - 18 in comparision with FGF - 2 and FGF - 10 [ J ]. J Biol Chem, 2002, 277 (9) :7493 - 7450

二级参考文献5

  • 1[1]Whitmore TE, Maurer MF, Sexson S, et al.Assignment of fibroblast growth factor 18 (fgf18) to human chromosome 5q34 by use of radiation hybrid mapping and fluorescence in situ hybridization[J]. Cytogenet Cell Genet, 2000,90:231～3.
  • 2[2]Ohuchi H, Kimura S, Watamoto M, et al.Involvement of fibroblast growth factor fgf 18-fgf8 signaling in specification of left-right asymmetry and brain and limb development of the chick embryo[J]. Mech Dev, 2000,95:55～66.
  • 3[3]Ellsworth JL, Berry J, Bukowski T, et al.Fibroblast growth factor-18 is a trophic factor for mature chondrocytes and their progenitors[J]. Osteoarthritis Cartilage, 2002,10:308～20.
  • 4[4]Liu Z, Xu J, Colvin JS,et al.Coordination of chondrogenesis and osteogenesis by fibroblast growth factor 18[J]. Genes Dev ,2002 ,16:859～69.
  • 5[5]Ohbayashi N, Shibayama M, Kurotaki Y.fgf18 is required for normal cell proliferation and differentiation during osteogenesis and chondrogenesis[J]. Genes ev ,2002 16:870～9.

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牙体牙髓牙周病学杂志
2006年 第5期

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