摘要
目的研究环胞苷和丙氧鸟苷联合用药的抗单疱病毒(herpes simplex virus,HSV)作用;方法用空斑抑制试验测定环胞苷和丙氧鸟苷单独或联合用药后抗 HSV 的半数抑制浓度(50%inhibitive concentration of HSV plaque formation,IC_(50)),应用图解法和分部抑制浓度(fractionalinhibitory concentration,FIC)指数,评价环胞苷和丙氧鸟苷联合应用的抗 HSV 活性;结果在组织培养中,环胞苷和丙氧鸟苷抑制 HSV-1的 IC_(50)分别为0.19μg/ml 和0.10μg/ml。当环胞苷和丙氧鸟苷联合应用时,抗 HSV 活性有明显协同作用,FIC 指数均小于0.75。环胞苷和丙氧鸟苷合用尚能减轻及延缓 HSV 耐药性的产生;结论环胞苷和丙氧鸟苷联合应用可能是治疗 HSV 眼部感染的一种有效方法.
Objective The study was designed to investigate the combined effect of cyclocytidine (CC) and ganciclovir (GCV) on herpes simplex virus-1 (HSV-1) in cell culture.Methods The 50% inhibition concentrations of HSV-1 plaque formation (IC_(50)) of CC,GCV alone and in combination were determined by the inhibitory test of plaque formation.The combined anti-HSV-1 effect of CC and GCV was evaluated by a graphic method and fractional inhibitory concentration (FIC) indexes.Results IC_(50) of CC and GCV was 0.19 and 0.1μg/ml,respectively.The combination of CC with GCV produced sig- nificantly synergistic activity against HSV-1 in cell culture.FIC indexes were all below 0.75.The com- bined therapy of CC and GCV can also decrease and delay the emergence of drug-resistant variants. Conclusion These results suggest that this combined therapy of CC and GCV may be a potentially ef- fective means in the management of patients with HSV-1 ocular infection.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1996年第1期25-28,共4页
Chinese Journal of Ophthalmology
关键词
药物疗法
人疱疹病毒
环胞苷
丙氧鸟苷
眼部感染
Drug therapy,combined
Tissue culture
Herpesvirus hominis
Cyclocytidine Ganciclovir
