摘要
建立了小麦中雪腐镰刀菌烯醇（NIV）和脱氧雪腐镰刀菌烯醇（DON）的高效液相色谱同时检测方法。样品采用乙腈-水（体积比85：15）混合溶剂进行提取．通过多功能净化柱（MFC）进行一次性净化，以C18柱为分离柱，水-乙腈-甲醇（体积比90：5：5）混合溶剂为流动相进行高效液相色谱分离和检测。在小麦样品中，本方法在0．2～5μg／g添加范围内的凹收率为87％～99％；相对标准偏差为1．5％～8．3％；DON和NIV的检出限分别为0．12和0、16μg／g（5／N=3）。
A HPLC method for simultaneous determination of deoxynivalenol （DON） and nivalenol （NIV） in wheat was developed. After extracting the sample with a mixture of acetonitrile - water（85 ： 15 by volume）, the extract was one-time purified by multifunctional clean-up column. DON and NIV were then separated and determined by reversed-phase HPLC with Symmetry^R C18 column and a mixture of water-acetonitrile- methanol（90 ： 5 ： 5 by volume） as mobile phase. The linear range for DON was 0. 5 - 10 mg/L with a correlation coefficient of 0. 998 8 and that for NIV was 0. 5 - 10 mg/L with a correlation coefficient of 0. 999 1. The deteetion limits for DON and NIV were 0. 12 and 0. 16μg/g （S/N= 3） , respectively. The recovery ranges tbr DON and NIV were 92% - 96% and 87% - 99% , respectively, and their standard deviation range was 1.5% -8.3%.
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第3期56-59,共4页
Journal of Instrumental Analysis
