摘要
以3个大豆品种的体细胞胚为受体,以PSY为目的基因,应用基因枪法对大豆进行了遗传转化,实验结果表明:不同大豆基因型的体细胞胚对抗性选择标记(Hyg)的敏感性存在极显著差异;高渗处理的培养基加入0.4mol/L甘露醇有利于PSY基因的导入;对已获得10株抗性再生苗,经PCR和PCR-Southern分析鉴定,有4株为阳性,初步证明外源基因PSY已整合到大豆的基因组中。
Using somatic embryogenesis of three varieties of soybean as acceptor, PSY gene were transformed into soybean via particle bombardment. The results showed： the sensitivity of somatic embryo of soybean with different genotypes to resistance selectiwe marker（Hyg） is significantly different; high osmotic pressure medium with 0.4mol/L mannitol was beneficial for the transformation of target gene. 10 resistant regenerated plants were obtained from the three varieties, 4 plants gave positive reaction in PCR and PCR-Southern analysis from the gene transferred plants,indicating that PSY has been transferred into genome of soybean.
出处
《大豆科学》
CAS
CSCD
北大核心
2006年第2期137-140,共4页
Soybean Science
基金
"国家植物转基因技术研究开发与中试基地建设"专项课题(项目编号J99-B-001)
关键词
基因枪
PSY基因
大豆
体细胞胚
遗传转化
Particle bombardment
PSY gene
Soybean
Somatic embryogenesis
Genetic Transformation
