摘要
目的探讨溴甲酚类试剂在肾脏疾病患者血清白蛋白(A lb)测定中的偏差。方法用溴甲酚绿(BCG)、溴甲酚紫(BCP)与免疫散射比浊法(ITM)测定肾病综合征和血液透析患者血清A lb,并对测定值作比较。结果肾病综合征患者血清A lb的BCG测定值偏高,最高差值达7.8 g/L,偏高值可用方程Y=0.678Xα2球蛋白-2.405表达。血液透析患者血清A lb的BCP测定值约偏低3.1 g/L。结论当肾病综合征患者血清α2球蛋白增高、A lb/球蛋白(A/G)<0.8时,α2球蛋白对BCG法测定有明显影响,BCP法则由于血液透析患者血清中的3-羧基-4-甲基-5-丙基-2-呋喃丙酸(CMPF)的影响导致测定值偏低。
Objective To investigate the deviation of serum albumin（Alb） in patient with renal in the determination by bromocresol green （BCG） or bromocresol purple （BCP） reagent. Methods The Alb in nephrotic syndrome and hemodialyzed patient was determined by BCG, BCP assay and compared with immuno-turbidimetric method （ITM）. Resuits In nephrotic syndrome patients, the level of serum Alb determined by BCG assay was higher than that determined by ITM and the top value of the difference was 7.8 g/L. In hemodialyzed patients, the result of BCP assay was lower about 3.1 g/L than ITM. Conclusions High concentration of ct2 globulin has obviously affected the Alb determination by BCG reagent especially when serum A/G 〈 O. 8 in nephrotic syndrome patients. In hemodialyzed patients, underestimated value appeared correlated to the 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid （CMPF） in the serum.
出处
《检验医学》
CAS
北大核心
2006年第3期231-233,共3页
Laboratory Medicine
关键词
白蛋白
溴甲酚绿
溴甲酚紫
肾病综合征
血液透析
Albumin
Bromocresol green
Bromocresol purple
Nephrotic syndrome
Hemodialysis
