摘要
采用自行设计的超声强化超临界CO2反相微乳萃取设备,以AOT为主表面活性剂,乙醇为助表面活性剂,研究了超声对超临界CO2反相微乳萃取人参皂甙的影响.试验发现,在萃取压力、温度、时间和CO2流量分别为24 MPa、45℃、4 h、2 L/h的条件下,超声强化AOT/乙醇/超临界CO2反相微乳的人参皂甙萃取率是以乙醇作夹带剂的超临界CO2萃取法的7.42倍、AOT/乙醇/超临界CO2反相微乳萃取法的2.30倍;人参皂甙萃取率随超声辐照时间的延长而上升,随超声功率的增大先升高后降低;在超声功率较低的条件下,低频超声比高频超声更有利于反相微乳的萃取,而在超声功率较高的条件下,高频超声则比低频超声更有利于反相微乳的萃取.并且超声的加入没有改变超临界CO2反相微乳萃取的选择性.超声对超临界CO2反相微乳萃取的强化效应可能归因于超声的机械波动效应和热效应.
With the self-designed interpolation ultrasound-enhanced supercritical CO2 reverse microemulsions （USCRM） extraction device, bis（2-ethylhexyl） sodium sulphosuccinate （AOT） as a main surfactant and ethanol as a co-surfactant, the effect of ultrasound on ginsenosides extraction by supercritical CO2 reverse microemulsions （SCRM） was studied. The experimental results show that at pressure 24 MPa, temperature 45℃, time 4 h and CO2 flow rate 2.0 L/h, the ginsenosides extraction yield by USCRM is 7.42 and 2.30 times that by ethanol/supercritical CO2 and by AOT/ethanol/supercritical CO2 reverse microemulsions, respectively. The ginsenosides extraction yield increases with ultrasonic irradia, tion time, and first increases and then decreases with increasing ultrasonic power. Low-frequency ultrasound is more beneficial to improve the ginsenosides yield than high-frequency one at a low power, but is opposite at a high power. Ultrasound does not change the total ginsenosides mass fraction of solid extract in SCRM. The effect of ultrasound in supercritical CO2 reverse microemulsions may be attributed to mechanical fluctuant and thermal effects.
出处
《江苏大学学报（自然科学版）》
EI
CAS
北大核心
2006年第3期202-206,共5页
Journal of Jiangsu University：Natural Science Edition
基金
广州市科技计划项目(穗科2002-27)
关键词
萃取
甙
超声
超临界CO2反相微乳
强化作用
extraction
ginsenosides
ultrasound
supercritical CO2 reverse microemulsions
enhanced effect
