摘要
建立了固相萃取-高效液相色谱法(SPE-HPLC)测定地表水中三嗪类化合物的方法。考察了4种不同固相萃取柱对三嗪类化合物的吸附效果,最终选择ENVI-18固相萃取柱用于萃取地表水中的三嗪类化合物;系统研究了环境水样中三嗪类化合物的最佳固相萃取条件,选择洗脱溶剂为甲醇,洗脱溶剂用量5m L,水样在萃取前不需要添加甲醇,不调节pH值。测定了方法的检测限,结果表明,扑草净、莠去津、西玛津、脱乙基莠去津、羟基化莠去津和脱异丙基莠去津的最低检测限依次为0.14μg/L,0.12μg/L,0.08μg/L,0.08μg/L,0.10μg/L和0.18μg/L。将该法应用于实际环境水样的分析测定,结果表明某湖水中扑草净的含量为(9.33±0.27)μg/L,某江水中莠去津和扑草净的含量分别为(5.28±0.43)μg/L和(7.12±0.54)μg/L。
A method was developed to monitor triazines in surface water using the combination of solid phase extraction （ SPE ） and high performance liquid chromatography. Four different SPE cartridges were tested for extracting six triazines, including atrazine （A）, simazine （S）, prometryn （ P）, desethylatrazine （ DEA）, 2-hydroxyatrazine （OHA） and desisopropylatrazine （DIA）, and finally ENVI-18 was selected as optimal. The method for solid phase extraction was further systematically studied for details. Optimal results of orthogonal design were determined as follows： pH 6, 5 mL methanol as eluting solvent, and no methanol added into water sample before extraction. The detection limits of six triazines were 0. 14 μg/L for P, 0. 12 μg/L for A, 0.08 μg/L for S, 0.08 μg/L for DEA, 0. l0 μg/L for OHA and 0.18 μg/L for DIA. This method was applied for environmental aquatic samples, and the results showed that the concentration of prometryn in a lake was detected as （9.33 ± 0.27） μg/L, as well as the concentrations of atrazine and prometryn in a river were detected as （5.28 ±0. 43） μg/L and （7. 12 ± 0.54） μg/L respectively.
出处
《色谱》
CAS
CSCD
北大核心
2006年第3期267-270,共4页
Chinese Journal of Chromatography
基金
国家自然科学基金项目(No.20477030)
上海市科委2005年重点研究资助项目(No.05JC14059
No.05dz22330)
关键词
固相萃取
高效液相色谱
三嗪类化合物
预富集
solid phase extraction （SPE）
high performance liquid chromatography （HPLC）
triazines
preconcentration
