摘要
目的:观察温肾方药对甲状腺功能减退症大鼠甲状腺功能及血清性激素水平的影响,并分析该影响是否有剂量依赖性,该方药与西药甲状腺片合用是否效果更好。方法:实验于2005-04/05在上海中医药大学附属龙华医院科技实验中心完成。①选用清洁级健康成年Wistar大鼠60只,雌雄各半。②取10只为正常对照组:正常饲养。给予其余50只大鼠每天灌胃0.4g/L他巴唑混悬液1.35mL(2.7mg/kg),持续45d。第46天,将造模后大鼠50只随机分为5组:模型组、甲状腺片组、温肾方小剂量组、温肾方大剂量组、温肾方合甲状腺片组,每组10只。③模型组:灌胃9.0mL/kg生理盐水;甲状腺片组:灌胃9.0mL/kg甲状腺片混悬液穴上海市实业联合集团长城药业有限公司生产,批号20051001,40mg/片;用生理盐水配成0.4g/L混悬液雪;温肾方小剂量组:灌胃9.0mL/kg半硫丸悬液(半硫丸,主要成分为硫磺;将硫磺和豆腐以1∶1.5的比例进行炮制;由上海中医药大学附属龙华医院药剂科制为胶囊,用生理盐水配成30g/L混悬液);温肾方大剂量组:18.0mL/kg半硫丸胶囊混悬液;温肾方+甲状腺片组:灌胃9.0mL/kg甲状腺片混悬液和9.0mL/kg半硫丸悬液,1次/d,共15d。④15d后取大鼠颈部血液,采用放射免疫法测定血清游离三碘甲状腺原氨酸、游离甲状腺素、促甲状腺激素、促卵泡激素、促黄体生成素、雌二醇、孕酮水平。⑤各组计量结果差异比较采用方差分析和q检验。结果:大鼠60只均进入结果分析。①温肾方对甲状腺功能减退症大鼠甲状腺功能的影响:各治疗组血清游离三碘甲状腺原氨酸、游离甲状腺素水平明显高于模型组(P<0.05～0.01),血清促甲状腺激素水平明显低于模型组(P<0.05～0.01)。温肾方大剂量组、甲状腺片组、温肾方+甲状腺片组血清游离三碘甲状腺原氨酸、游离甲状腺素水平明显高于温肾方小剂量组(P<0.01),血清促甲状腺激素水平明显低于温肾方小剂量组(P<0.05～0.01)。温肾方+甲状腺片组血清游离三碘甲状腺原氨酸、游离甲状腺素水平明显高于其他治疗组及模型组(P<0.01),血清促甲状腺激素水平明显低于其他治疗组及模型组(P<0.05～0.01)。②温肾方对甲状腺功能减退症大鼠血清性激素水平的影响:除温肾方小剂量组对雄性大鼠促黄体生成素、雌性大鼠促卵泡激素外,各治疗组大鼠血清性激素水平明显高于模型组(P<0.01);除温肾方大剂量组雌性大鼠促黄体生成素、孕酮外,温肾方大剂量组、甲状腺片组、温肾方+甲状腺片组大鼠血清促卵泡激素、促黄体生成素、雌二醇、孕酮水平均明显高于温肾方小剂量组(P<0.05～0.01);其中温肾方+甲状腺片组与其他治疗组比较,均差异明显(P<0.05～0.01)。结论:温肾方可调节甲状腺功能减退症血清性激素水平,改善甲状腺功能,且该作用存在剂量依赖性,温肾方与甲状腺片合用效果更好。
AIM： To observe the effect of wenshenfang on thyroxine metabolism and sexual hormone in rats with hypothyroidism, and study whether there is dosage dependence or better effect in combination of wenshenfang and thyroid tablet.
METHODS： The experiment was carried out in Technological Experimental Center of Longhua Hospital affiliated to Shanghai University of Traditional Chinese Medicine from April to May 2005. ①Sixty adult clean healthy Wistar rats, female and male in half, were selected. ②Ten of the rats, . chosen as control group, were fed routinely. The other 50 rats were infused 0.4 g/L tapazole suspension 1.35 mL （2.7 mg/kg） for 45 days. On the 46^th day, the rats, which were made hypothyroidism, were randomly divided into 5 groupS： model group, thyroxin group; small and large dosage group of wenshenfang, and integrated wenshenfang and thyroxin group, with 10 rats in each group. ③The rats in the model group were infused 9.0 mL/kg Sodium Chloride; the rats in the thyroxin group were infused 9.0 mL/kg thyroxin suspension （made by Changcheng Medicine Co., Ltd. of Shanghai Industry United Holdings, No.20051001, 40 mg per plate; 0.4 g/L suspension was made with Sodium Chloride）; the rats in the small dosage group of wenshenfang with 9.0 mL/kg banliu wan suspension （the major component of banliu wan was sulphur; sulphur was prepared with bean curd in the proportion of 1：1.5 and made gelatin capsule in Pharmacy of Longhua Hospital affiliated to Shanghai University of Traditional Chinese Medicine, 30 g/L suspension was made with Sodium Chloride）; the rats in the large dosage of wenshenfang with 18.0 mL/kg banliu wan suspension; the rats in the integrated group with 9.0 mL/kg thyroxin suspension and 9.0 mL/kg banliu wan suspension, once per day for 15 days. ④Fifteen days later, the blood was collected from the neck of the rats to detect serum free triiodothyronine （FT3）, free thyroxine （FT4）, thyroid-stlmulating hormone （TSH）, follicle-stimulating hormone （FSH）, luteinizing principle （LH）, estradiol （E2）, and pregnendione （P） by radioimmunoassay. ⑤The data were compared with X^2 and q-test. RESULTS： All the 60 rats were involved in the result analysis. ①Effect of wenshenfang on thyroid function of the rats with hypothyroidism： The content of serum FT3 and FT4 in each treated group was significantly higher than those in the model group （P 〈 0.05-0.01）, while the serum TSH was obviously lower than that in the model group （P 〈 0.05-0.01）. The content of serum FT3 and FT4 in large dosage group, thyroxin group and the integrated group was all significantly higher than those in small dosage group （P 〈 0.01）, while the content of serum TSH was markedly lower than that in small dosage group （P 〈 0.05-0.01）. The content of serum FT3 and FT4 in the integrated group were all significantly higher than those in other treated groups and model group （P 〈 0.01）. while the content of serum TSH was significantly lower than that, in other treated groups and model group （P 〈 0.05-0.01）. ② Effect of wenshenfang on serum sexual hormones： The content of serum sexual hormones in treated groups were all significantly higher than those in the model group （P 〈 0.01） except for the content of LH of male rats and FSH of female rats in small dosage group. The content of serum FSH, LH, E2 and P in large dosage group, thyroxin group and the integrated group were all significantly higher than those in small dosage group （P 〈 0.05-0.01）, except for LH and P in large dosage group, among which there were significantly differences between the integrated group and the other treated groups （P 〈 0.05-0.01）.
CONCLUSION： Wenshenfang can adjust the serum sexual hormones in hypothyroidism and improve the thyroid function; moreover, the effect has dosage dependence. Combined usage of wenshenfang and thyroxin has better effect.
出处
《中国临床康复》
CSCD
北大核心
2006年第19期152-154,共3页
Chinese Journal of Clinical Rehabilitation
基金
上海市重点学科建设项目资助(T0304)~~
