摘要
背景:补气通络方有补气行血、通络散结、生肌长肉、活血通络之功效,可促进损伤局部血液循环,改善神经营养,从而促进周围神经轴突再生,促进神经传导功能恢复。目的:观察不同制剂的补气通络方中药对周围神经损伤后神经功能恢复的作用,并与维生素B1,B6的效果进行比较。设计:随机对照动物实验。单位:广州中医药大学第一附院骨科。材料:取封闭清洁级Wistar大鼠48只,统一在右坐骨神经造模后,随机分成4组(n=12),补气通络胶囊组,补气通络注射液组,维生素组,空白组。方法:所有大鼠行右坐骨神经横形切断后即刻原位吻合术造模,制成周围神经损伤模型。术后第3天起给药,1次/d:①补气通络胶囊组大鼠灌胃补气通络胶囊中的药末(含黄芪、人参(新开河参)、当归、川芎、丹参等中药,用生理盐水稀释),给药量生药0.9g/(kg·d)。②补气通络注射液组大鼠腹腔注射补气通络注射液0.9g/(kg·d)。③维生素组大鼠灌胃含维生素B1和维生素B61.5g/L混悬液15mg/(kg·d)。④空白组大鼠生理盐水0.01mL/g灌胃。主要观察指标:给药后4,8,12周每组随机抽取4只,记录双侧神经传导速度,并以同一大鼠的受伤侧神经传导速度除以正常侧神经传导速度值作为神经传导速度恢复率。结果:48只全部进入结果分析。①在各时间点补气通络方两个治疗组的神经传导速度均显著快于维生素组和空白组(P<0.05,0.01),维生素组神经传导速度较空白组快(P<0.05)。②在各时间点补气通络方两个治疗组的神经传导速度恢复率均显著高于维生素组和空白组(P<0.05,0.01),维生素组高于空白组(P<0.05)。结论:胶囊和针剂的补气通络方中药对周围神经损伤后神经功能恢复均促进作用,效果优于维生素B1和B6。
BACKGROUND： Buqi tongluo prescription, which is characterized by invigorating qi and promoting blood circulation, dredging collaterals and dispersing stagnation, promoting tissue regeneration, and promoting blood circulation to remove obstruction in the collateral, can promote blood circulation at local injured part and ameliorate nutrien of nerve so as to accelerate axonal regeneration of peripheral nerve and functional recovery of nerve conduction.
OBJECTIVE： To observe the effect of buqi tongluo prescription of various agents on recovery of nerve function after peripheral nerve injury and compare the results with the combination of vitamin B1 and B6.
DESIGN： Randomized controlled animal study.
SETTING： Department of Orthopaedics of the First Hospital Affiliated tO Guangzhou University of Traditional Chinese Medicine.
MATERIALS： A total of 48 Wistar rats of confined clean grade were selected in this study. After modeling on right sciatic nerve, rats were randomly divided into buqi tongluo capsule group, buqi tongluo parenteral solution group, vitamin group and blank group with 12 in each group.
METHODS： All rats were cut their right sciatic nerve laterally, and then anastomosis in situ was used immediately to establish models of peripheral nerve injury. Administration began on the third day after operation once a day. ① Rats in buqi tongtuo capsule group were perfused with powder of buqi tongluo capsule （including huangqi, renshen, danggui, chuanxiong and danshen which was diluted with saline） of 0.9 g/（kg ·d） raw drugs （ig）. ② Rats in buqi tongtuo parenteral solution group were injected with 0.9 g/（kg ·d） buqi tongluo parenteral solution （ip）. ③ Rats in vitamin group were perfused with 15 mg/（kg·d） suspension （1.5 g/L） of vitamin B1 and B6 .④ Rats in blank group were perfused with 0.01 mL/g saline.
MAIN OUTCOME MEASURES： Four rats from each group were selected for 4, 8 and 12 week time points after administration to record bilateral nerve conduction velocity. Recovery rate was regarded as the ration between nerve conduction velocity at the injured side and nerve conduction velocity at the normal side.
RESULTS： A total of 48 rats were involved in the final analysis. ① At each time point, nerve conduction velocity in two buqi tongluo prescription groups were faster than that in vitamin group and blank group （P 〈 0.05, 0.01）, and that in vitamin group was faster than that in blank group （P 〈 0.05）.② At each time point, recovery rate of nerve conduction velocity in two buqi tongluo prescription groups was higher than that in vitamin group and blank group （P 〈 0.05, 0.01）, and that in vitamin group was higher than that in blank group （P 〈 0.05）.
CONCLUSION： Both buqi tongluo capsule and buqi tongluo parenteral solution can promote recovery of nerve function after peripheral nerve injury, and the effect is superior to that of vitamin B1 and B6.
出处
《中国临床康复》
CSCD
北大核心
2006年第19期179-181,共3页
Chinese Journal of Clinical Rehabilitation
基金
广东省中医药局资助项目(97197)~~
