摘要
采用磷酸铅沉淀技术对小麦（Ｔｒｉｔｉｃｕｍ ａｅｓｔｉｖｕｍ ）珠心细胞衰退过程进行了ＡＴＰ酶的超微细胞化学定位。初始衰退的珠心细胞，ＡＴＰ酶只定位于细胞膜上，其它部位未见有ＡＴＰ酶活性。衰退中期的珠心细胞，细胞膜上ＡＴＰ酶活性减弱并逐渐消失；细胞核染色质和细胞质中一些细胞器上存在ＡＴＰ酶活性。在严重衰退的珠心细胞中，只在细胞核染色质上存在ＡＴＰ酶活性。珠心细胞的细胞核以两种方式衰退。衰退的细胞核染色质碎片仍存在ＡＴＰ酶活性，并向胚囊方向转移。推测小麦珠心细胞衰退过程中细胞膜上ＡＴＰ酶变化反映了珠心细胞生理状态转变；
Ultracytochemical localization of ATPase activity was carried out using a lead phosphate precipitation technique in the nucellar cells of wheat during degeneration.ATPase was only localized on the plasma membranes of nucellar cells at the early degenerative stage, then decreased and disappeared at the mid degenerative stage. Meantime it was also observed in the nuclear chromatin and some cytoplasmic organelles. ATPase activity was only observed in the nuclear chromatin in the extremely degenerated nucellar cells.Two patterns of unclear degeneration was found in the degenerated nucellar cells.A lot of small fragments with ATPase from the degenerated nuclei moved toward the embryo sac.It is suggested that the change of ATPase activity on the plasma membranes is related to the physiological change of nucellar cells, and that in the nuclear chromatin is associated with the stages movement of chromatin during the process of nucellar cells degeneration.
基金
国家自然科学基金
关键词
小麦
麦心细胞
衰退
腺苷三磷酸酶
超微细胞化学
Triticum aestivum
Nucellar cell
Degeneration
ATPase
Ultracytochemical localization
