摘要
目的研究胃癌患者细胞表面黏附分子CD44在外周血淋巴细胞中的表达及临床意义. 方法根治术的胃癌患者49例.外周血淋巴细胞中CD44的表达应用流式细胞免疫学方法检测,并与正常对照（n=25）进行比较. 结果胃癌患者外周血淋巴细胞中CD44的表达均明显高于正常对照[（437.1±16.3） vs （353.3±18.1）,P＜0.01],手术后2周（381.9±18.7）和12个月（381.5±21.6）表达降低,但仍高于对照（P＜0.05）.胃癌Ⅲ～Ⅳ期CD44的表达明显高于Ⅰ～Ⅱ期[（479.1±24.6） vs （423.2±19.5）,P＜0.05],胃癌有转移患者CD44表达均高于无转移[（462.1±19.2） vs （428.4±19.6）,P＜0.01],但不同组织类型的CD44表达无显著性差异（P＞0.05）. 结论胃癌患者外周血淋巴细胞中CD44的表达水平明显高于正常对照,且手术后可以明显下降,其表达水平与胃癌的进展情况及有无转移、预后有一定关系.
Objective To investigate the expression of peripheral blood CD44 in gastric cancer. Methods Data of forty-nine patients with gastric cancer undergoing gastrectomy were reviewed, and 25 normal donors served as controls. The expression of peripheral blood CD44 detected by flow-cytometry, Results The contents of CD44 in peripheral blood lymphocyte in patients with gastric cancer were significantly higher than in normal controls [ （437, 1 ± 16.3） vs （353.3 ± 18.1 ）, P〈0.01 ], But the level of CD44 decreased （P〈0,05） after operation in 2 weeks （381.9 ± 18,7） and 12 months （381.5 ± 21.6）, Distinctly statistic difference was showed in CD44 between Ⅲ- Ⅳ stages and Ⅰ -Ⅱ stages [（479, 1 ± 24.6） vs （423, 2 ± 19.5） ,P〈0.05], Gastric cancer with metastasis or distance metastasis showed higher expression of CD44 than that without metastasis or distance metastasis [ （462, 1 ± 19.2） vs （428,4 ± 19.6） ,P〈0.01], No difference in CD44 contents was observed in different histopathological types in gastric cancer （P〉0.05）, Conclusion The contents of CD44 in peripheral blood lymphocyte in gastric cancer are significantly higher, and CD44 lower after operation. The CD44 expression in gastric cancer is an important way to differentiate benign from malignant tumors, and to detect invasive or metastasis and estimate prognosis of gastric cancer.
出处
《山西医科大学学报》
CAS
2006年第3期242-244,共3页
Journal of Shanxi Medical University
关键词
胃肿瘤
血液
抗原
CD44
流式细胞术
免疫
细胞
stomach neoplasms
blood
antigens,CD44
flow-cytometry
immunity,eellular
