摘要
目的:检测从食物中毒中分离的金黄色葡萄球菌的肠毒素基因,明确肠毒素的基因型。对所分离的金黄色葡萄球菌进行药敏试验,检测其耐药性。方法:应用PCR扩增金黄色葡萄球菌SEA-SEJ基因,电泳检测扩增结果;用VITEK32检测药敏结果。结果:检测到金黄色葡萄球菌的肠毒素基因SEG和SEI,菌株的耐药性不强。结论:金黄色葡萄球菌的肠毒素SEG和SEI可能是这起食物中毒的因子。
Objective： To detect and type staphylococcal enterotoxin in Staphylococcus aureus isolates from food poisoning. Methods：Toxin genes were amplified by PCR, and the different amplicons in size detected by eleetrophoresis. The antibiosis were determined by VITEK32. Results： Entevo genes SEG and SEI were detected in S. aureus isolates. Conclusion： The SEG and SEI are possible causes for this food poisoning.
出处
《中国卫生检验杂志》
CAS
2006年第6期665-666,688,共3页
Chinese Journal of Health Laboratory Technology
