摘要
利用紫外、荧光和粘度等方法研究了含不同配体的钌(II)配合物[Ru(phen)2CImP]2+(CImP=3,4-二羟基-咪唑并[4,5-i][1,10]邻菲咯啉)和[Ru(phen)2TPPZ]2+(TPPZ=四吡啶[3,2-a:2',3'-c:3'',2''-h:2''',3'''-j]吩嗪)与DNA的作用机制,并研究了配合物与Zn2+配合后荧光性质变化.结果表明[Ru(phen)2TPPZ]2+与DNA以插入模式作用,而[Ru(phen)2CImP]2+与DNA则以沟面结合模式作用.向配合物溶液中滴加Zn2+后,配合物[Ru(phen)2TPPZ]2+和[Ru(phen)2CImP]2+均可以与Zn2+形成双核配合物[Ru(phen)2(TPPZ)Zn]4+和[Ru(phen)2(CImP)Zn]4+,配合物的荧光减弱.与DNA作用后,配合物仍可以与Zn2+配位形成双核配合物,但[Ru(phen)2(TPPZ)Zn]4+保持插入模式与DNA作用,配合物的荧光减弱.而[Ru(phen)2(CImP)Zn]4+与DNA则由沟面结合改为插入结合,配合物的荧光增强.
The binding modes of polypyridyl 3,4-dihydroxyl-imidazo[4,5-i]（1,10-phenanthroline） ruthenium（II） complexes [Ru（phen）2CImP]^2＋ （CImP： and [Ru（phen）2TPPZ]^2＋ （TPPZ： tetrapyrido[3,2-c：2＇, 3＇-e：3＂,2＂-j：2＇＂,3＇＂-l]phenazine） with calf thymus DNA have been investigated by spectroscopic and viscosity methods. The luminescent properties of coordinated complexes with Zn^2＋ were also studied. The experimental results indicated that [Ru（phen）2TPPZ]^2＋ bound to DNA by intercalation, while [Ru（phen）2CImP]^2＋ bound to DNA by groove binding mode. Both complexes could be coordinated with Zn^2＋ to form the heterobimetallic complexes [Ru（phen）2（TPPZ）Zn]^4＋ and [Ru（phen）2（CImP）Zn]^4＋, and the emission intensity of complexes decreased with increasing the amount of Zn^2＋. After binding to DNA, both original complexes could be coordinated with Zn^2＋ to form the heterobimetallic complexes, and [Ru（phen）2（TPPZ）Zn]^4＋ kept binding to DNA by intercalation, with the emission intensity decreased, while the binding mode of [Ru（phen）2（CImP）Zn]^4＋ was changed from groove binding to intercalation, with increase of the emission intensity.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2006年第10期968-974,共7页
Acta Chimica Sinica
基金
广东省自然科学基金博士启动基金(No.04300882)
深圳市科技计划(No.200328)资助项目.
