摘要
目的:建立柴胡属植物地上部分中槲皮素、异鼠李素、山奈素HPLC的含量测定方法。方法:伊利特-C18柱(250 mm×4．6 mm,5μm),以甲醇-水(60:40),磷酸调节pH至3．0为流动相,流速:1．0 ml·min-1,检测波长:360 nm。结果:槲皮素在0．70-5．57μg(r槲=0．999 7),异鼠李素在0．16～1．30μg(r异=0．999 2),山奈素在0．09-0．71μg(r山=0．999 4)范围内呈良好的线性关系。平均回收率分别为97．0％,96．8％,97．4％,RSD分别为2．2％,2．2％,1．1％。结论:本法专属性强,灵敏度高,重现性好,适于黄酮类成分的分离与测定。
Objective. To establish a HPLC method for determination the content of Quercetin, isorhamnetin and Kaempferol in Bupleurum. L. Method： the yilite C18 column（250 mm× 4.6 mm, 5 μm）was used, the mobile phase consisted of methanol-water（60 ：40） adjust pH to 3.0 with phosphoric acid, the flow rate was 1.0 ml.min^-1, the detection wavelength was at 360 nm. Result： the linear ranges of quercetin, isorhamnetin and kaempferol were 0. 70-5.57 μg （ r = 0. 999 7 ）, 0.16-1.30 μg（ r = 0.999 2 ）, and 0.09- 0.71 μg（ r =0.999 4） respectively, the both showed good＇linear correlation, the average recoveries were 97.0% （ RSD = 2.2% ）, 96.8% （RSD =2.2% ） and 97.4% （RSD = 1.1% ） respectively. Conclusion： the method is sensive and rapid, it can be used for quality control of Bupleuruml. L
出处
《中国药师》
CAS
2006年第6期501-503,共3页
China Pharmacist
