摘要
目的 建立HPLC测定肠参定位片中氧化苦参碱含量的方法.方法采用Agillent Zorbax SB-C18色谱柱(250mm ×4.6mm,5μm),流动相:0.043mol/L SDS溶液(含1/15 mol/L H3PO4-1/15 mol/L KH2PO4)-乙腈(76:24),流速:1.0ml/min,检测波长:220nm,柱温:室温.结果氧化苦参碱在0.25~2.00μg范围内具有良好的线性关系(r=0.9999),氧化苦参碱的平均加样回收率为100.04%,RSD=0.60%(n=5).结论该方法简便,准确,灵敏度高,重复性好,可用于肠参定位片的质量控制.
Objective To establish a HPLC method for determination of Oxymatrine in Changshendingweipian. Methods The HPLC system consisted of Agillent Zorbax SB - C18 column (250mm ×4. 6mm, 5μ m), 0. 043mol/L SDS - CH3CN (including 1/ 15 mol/L H3PO4 -1/15 mol/L KH2PO4) (76: 24) mixture as a mobile phase, detection wavelength at 220 nm, the flow rate was 1.0 ml/min. Results The linear range of Oxymatrine was 0. 25 - 2. 00μg, r = 0. 9999, the average recovery was 100.04%, RSD =0. 60% (n =5). Conclusions The method is simple, the result is accurate, higher sensitivity and the reproducibility is good. This method can be used for the determination of Changshendingweipian.
出处
《锦州医学院学报》
2006年第2期43-45,共3页
Journal of Jinzhou Medical College