摘要
以无花果良种布兰瑞克和紫果１号为材料，其匀浆滤汁经有机溶剂沉淀、超滤等方法处理。得到无花果蛋白酶粉。酶比活力为１５．３４ｕ／ｍｇ·ｐｒｏｔｅｉｎ，纯化倍数为１１．６８％，酶产率为１．５％，酶活力回收率为２７．４％，酶最适ｐＨ为８．０，在ｐＨ５～１０范围内处理０．５ｈ活性稳定，酶最适温度为４０℃，在温度５０℃以内活性稳定。酶具有琉基蛋白酶特性，能被半胱氨酸和ＥＤＴＡ激活，而被ＨｇＣｌ２抑制。
Uaing fine figs-Buran Ruiki and purple-fruit-1 as the material,treating its scattered pulp and filtered juice through preipitation in organic solvent,ultra-filtration,etc,we got the powder of fig proteolyfic enzyme.Its specific activity was 15.34 u/mg,protein,its purifioat-ion ploidy reached 11.68 percent.The nate of enzyme production was 1.5 percent,and therecovery ratio of enzytne activity was 27.4 percent.The enzyme exhibited stable activity atpH5～10 for haif an hour with the optimum pH of 8.0.It had stable activity at the tempera-ture below 50℃with the optimum of 40℃.The enzyme had the properties of lnercapto-proteinase.It could be activated by cysteine and EDTA,but inactivated by Hgcl2.
出处
《江西农业大学学报》
CAS
CSCD
1996年第1期46-50,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
江西省科委"八.五"重点攻关课题
