摘要
目的:探讨Survivin基因在非小细胞肺癌(NSCLC)中的表达,以及与P53,Bcl-2蛋白表达的相互关系。方法:应用免疫组织化学法(SP)检测80例NSCLC肿瘤组织、20例肺良性病变组织中Survivin蛋白、P53蛋白、Bcl-2蛋白表达情况,并将结果进行了相关分析。结果:Survivin蛋白在肺良性病变组织中不表达,在61.3%(49/80)的NSCLC组织中有表达,且Survivin的表达与肺癌患者的TNM分期有关,但与肺癌的细胞类型、分化程度及淋巴结转移无明显关系;肺癌组织p53蛋白阳性表达率55.0%(44/80),与肺癌的TNM分期及淋巴结转移有关,肺良性病变组织中无p53蛋白表达;肺癌组织Bcl-2蛋白阳性表达率50.0%(40/80)明显高于肺良性病变组织的10.0%(2/20),其中鳞癌组织的表达率62.2%,明显高于腺癌组织的表达率34.3%(P<0.05);肺癌组织中P53,Bcl-2蛋白与Survivin蛋白表达显著相关(P<0.05)。结论:Survivin蛋白在肺癌组织中表达上调,提示该基因对NSCLC的发生发展起重要作用。Survivin基因有望成为肺癌基因治疗的新靶点;Survivin蛋白表达与肺癌的TNM分期密切相关,提示可作为判断病情和评价预后的指标。Sur vivin蛋白的表达与p53蛋白、Bcl-2蛋白的表达均呈正相关,三者在肺癌的发生中可能起协同作用。
Objective ：To study the expression of survivin and its relationship with the expression of P53, Bcl - 2 in non small cell lung cancer （NSCLC）. Methods：Expression of the survivin, p53 and Bcl -2 proteins was evaluated by immunohistochemical assay in 80 NSCLC tumor samples, 20 inflammatory lung lesions. Results：Expression of survivin protein was detected in a significantly greater proportion of NSCLC （61.3%） than in inflammatory lung lesions （0.0%） （P 〈 0.05 ）; The expression of survivin correlated with TNM stages, and there was no relationship between survivin expression and histologic type , tumor differation and lymphnede metastasis . The expression of p53, was significantly higher in lung cancers （ 55.5% ） than in inflammatory lung lesions （0.0%） （ P 〈0.05）, and correlated with TNM stages and lymphnode metastasis. The expression of Bcl - 2, was significantly higher in lung cancers （50.0%） than in inflammatory lung lesions （ 10.0 % ）, and was significantly higher in squamous cancers （62.2%） than in adenocarcinomas （ 34.3% ） （ P 〈 0.05 ）. The expression of survivin correlated with p53, Bcl - 2 expression. Conclusion： The upregulation expression of survivin in NSCLC suggested that survivin may play a role in the pathway of carcinogenesis and may be identified as a potential therapeutic target in NSCLC. Expression of survivin correlated with TNM stages, and might be used to evaluate prognosis. The close relationships between the expression of survivin and p53, Bcl - 2 indicate that they might play synergetic roles in the process of carcinogenesis of NSCLC.
出处
《现代肿瘤医学》
CAS
2006年第6期678-680,共3页
Journal of Modern Oncology
