摘要
目的:探讨不同中药通过抗氧自由基作用对急性肺损伤(AL I)的影响。方法:60只纯种W istar大鼠随机分为A 1组(油酸+生理盐水注射液后24 h)、A 2组(盐水后36 h)、B 1组(油酸+维生素C注射液后24 h)、B 2组(油酸+维生素C后36 h)、C 1组(油酸+参脉注射液后24 h)、C 2组(油酸+参脉注射液后36 h)、D 1组(油酸+葛根素注射液后24 h)、D 2组(油酸+葛根素注射液后36 h)、E 1组(油酸+清开灵注射液后24 h)、E 2组(油酸+清开灵注射液后36 h)10组,每组6只。分别检测血浆丙二醛(M DA)、总超氧化物歧化酶(T SOD)、谷光甘肽(G SH)和谷光甘肽过氧化物酶(G SH Px)来评定氧自由基水平;用肺系数评定急性炎症和肺水肿程度;用右上叶肺组织光镜下观察炎症浸润程度。结果:①肺系数:D 1组24 h最高;②M DA:C 1组24 h最高,36 h A 2组和C 2组高于B 2组,D 2组最低;③T SOD:24 h和36 h D 1、D 2组、E 1、E 2组水平较低;④G SH:24 h C 1组最高,36 h B 2和D 2组最低;⑤G SH Px:36 h B 2组低于其他组;⑥光镜下病理检查见炎细胞浸润与肺泡充血水肿程度依次为:D 1组>A 1组>C 1组>B 1组>E 1组;A 2组>B 2组>C 2组>E 2组>D 2组。结论:维生素C、参麦、葛根素、清开灵注射液均是抗氧自由基药物,参脉注射液降低M DA和升高G SH的作用24 h以后较强;葛根素和清开灵注射液升高T SOD的作用较维生素C、参脉注射液略弱。
Objective： To investigate the effects of different anti-oxygen free radicals of various traditional Chinese drugs on acute lung injury （ALI）. Methods： Sixty Wistar rats were randomly divided into ten groups with 6 animals in each group： group A1, 24 hours after oleic acid ＋ normal saline （NS） injection; group A2, 36 hours after NS injection only; group B1, 24 hours after oleic acid ＋ vitamin C （VC） injection; group B2, 36 hours after oleie acid ＋ VC injection; group C1, 24 hours after oleic acid ＋ Shenmai injection （参脉注射液）; group C2, 36 hours after oleic acid ＋ Shenmai injection; group D1, 24 hours after oleic acid ＋ puerarin injection （葛根素注射液） ; group D2, 36 hours after oleic acid ＋ puerarin injection; group E1, 24 hours after oleic acid ＋ Qingkailing injection （清开灵注射液） ; group E2, 36 hours after oleie acid H-Qingkailing injection. The levels of free oxygen radicals were evaluated by determination of malondialdehyde （MDA）, total superoxide dismutase （T-SOD）, glutathione hormone （GSH）, and glutathione peroxidase （GSH-Px）. The degrees of acute inflammation and lung edema were evaluated by detection of lung coefficient. The degree of the inflammatory cell infiltration was observed under light microscope pathologically, using the tissue from apex of right lung. Results ： ①Lung coefficient ; The highest level at 24 hours was reached in group D1.②MDA： The highest level at 24 hours was reached in group C1, but at 36 hours, the levels of group A2 and group C2 were higher than that of the group B2. ③ T -SOD： The levels at 24 hours and 36 hours were lower in group D1, D2 and E1, E2 than other groups. ④GSH： Group C1 was the highest at 24 hours, and the group B2 and group D2 were lower at 36 hours. ⑤GSH-Px： Group B2 at 36 hours was the lowest of all the groups. ⑥ The degrees of lung congestion and edema ranked （from serious to light） as group D1〉A1〉C1〉B1〉E1; group A2〉B2〉C2〉E2〉D2. Conclusion： VC, Shenmai, puerarin, and Qingkailing injections are all the drugs for anti-oxygen free radicals. Shenmai injection is stronger than others in lowering MDA and increasing GSH after 24 hours ; puerarin and Qingkailing injections are weaker than VC and Shenmai injections in increasing T-SOD.
出处
《中国中西医结合急救杂志》
CAS
2006年第3期146-149,F0002,共5页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
吉林省教育厅科研基金资助项目(20051090)
关键词
中草药
急性肺损伤
氧自由基
大鼠
traditional Chinese medicinal herbs
acute lung injury
oxygen free radicals
