期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

荧光光谱法测定外周血DNA对恶性肿瘤的筛选价值

Value of peripheral blood DNA fluorscence spectra in the filtration of malignancy
下载PDF
导出
摘要 目的评价荧光光谱法测定外周血DNA对恶性肿瘤的筛选价值。方法提取恶性实体瘤、白血病、骨髓增生异常综合征(MDS)及正常人外周血DNA各50份,应用镧系离子荧光光谱分析法检测其荧光强度。结果恶性实体瘤、白血病、MDS及正常人外周血DNA荧光强度分别为:345.645±28.004、322.988±43.530、308.477±33.834、276.152±29.442,3组恶性疾病患者外周血DNA荧光强度均明显高于正常人(P<0.001),且3组之间荧光强度有显著性差异(P<0.05)。结论外周血DNA荧光检测对于恶性肿瘤具有一定的筛选价值,且可提高早期诊断率。 Objective To evaluate the value of peripheral blood DNA in the filtration of malignancy. Methods Extracting the peripheral blood DNA of 50 patients with various types of malignant solid neoplasias, leukemia, myelodysplastic syndrome（MDS） respectively and healthy individuals , then detecting the fluorescence intensity by lanthanode fluorescence spectra analysis. Results The mean DNA fluorescence intensity in the malignant solid neoplasias, leukemia, MDS were 345. 645 ±28. 004, 322.988 ±43. 530 and 308.4 ± 33. 834, respectively. It was obviously higher than that in clusions Peripheral blood DNA Fluorescence can rise the rate of the earlier diagnosis. the healthy individuals （ 276. 152 ± 29.442 ） （ P〈0.001 ）. Conspectra analysis is valuable for the filiration of malignancy, and
作者 顾晓怡 姜藻 何华 王羚郦 邓立春
机构地区 东南大学附属中大医院肿瘤科 中国药科大学药物分析系 东南大学附属江阴医院肿瘤科
出处 《现代医学》 2006年第3期166-169,共4页 Modern Medical Journal
基金 江苏省自然科学基金资助项目(KB2003080)
关键词 外周血DNA 恶性实体瘤 白血病 骨髓增生异常综合征 荧光光谱 镧系离子 peripheral blood DNA malignant solid neoplasias leukemia myelodysplastic syndrome fluo-rescence spectra lanthanide
分类号 R33 [医药卫生—人体生理学] R73 [医药卫生—肿瘤]
  • 相关文献

参考文献10

  • 1LEON S A,SHAPORO B,SKLAROFF D M,et al.Free DNA in the serum of cancer patients and the effect of therapy[J].Cancer Res,1977,37 (3):646-650
  • 2YONUSCHOT G,MUSHRUSH G W.Terbium as a fluorescent probe for DNA and chromatin[J].Biochemistry,1975,14(8):1677-1681.
  • 3杭建峰,吴英松,李明.时间分辨荧光免疫分析的研究进展及应用[J].热带医学杂志,2004,4(3):340-343. 被引量：47
  • 4SIDRANSKY D.Nucleic acid-based methods for the detection of cancer[J].Science,1997,278 (5340):1054-1059.
  • 5FOURNIE G J,LAMBER P H,MIESCHER P A.Release of DNA incirculating blood and induction of anti-DNA antibodies after injection of bacterial lipopoly-saccharides[J].J Exp Med,1974,140 (5):1189-1206.
  • 6GOEBEL G,ZITT M,MULLER HM.Circulating nucleic acids in plasma or serum (CNAPS) as prognostic and predictive markers in patients with solid neoplasias[J].Dis Markers,2005,21 (3):105-20.
  • 7CHERNITSKII E A,GRIGOROVICH N A,SLOBOZHANINA E I,et al.On the changes in the UV fluorescence of blood serum in tumor cases[J].Dokl Akad Nauk Bssr,1982,26:372-375
  • 8LEINER M J,SCHAUR R J,DESOYE G,et al.Fluorescence topography in biology.Ⅲ:Characteristic deviations of tryptophan fluorescence in sera of patients with gynecological tumors[J].Clin Chem,1986,32 (10):1974-1978.
  • 9SOZZI G,CONTE D,LEON M,et al.Quantification of free circulating DNA as a diagnostic marker in lung cancer[J].J Clin Oncol,2003,21 (21):3902-3908.
  • 10ZIEGLER A,ZANGEMEISTER-WITTKE U,STAHEL R A,et al.Circulating DNA:a new diagnostic goldmine?[J].Cancer Treat Rev,2002,28 (5):255-271.

二级参考文献48

  • 1游冬青,陈杞.镧系离子时间分辨荧光检测法在核酸分析技术中的应用[J].光电子技术与信息,1994,7(5):41-44. 被引量：5
  • 2[1]Uehar M, Arai Y, Watanabe's, et al. Compaarison of plasma and urinary phytoestrogens in Japanese and Finnish women by time-resolved fluoroimmunoassay[J] . Biofactors, 2000; 12(1 -4): 217 -225.
  • 3[2]Wang GJ, Lapcik O, Hampl R, et al. Time-resolved fluoroimmunoassay of plasma daidzein and genistein[J]. Steroids, 2000;65(6): 339 - 348.
  • 4[3]L'homme R, Brouwers E, Al-Maharik N, et al. Time-resolved fluoroimmunoassay of plasma and urine O-desmethylangolensin[J].Steroid Biochem Mol Biol, 2002; 81 (4 - 5): 353 - 361.
  • 5[4]Yamada H, Satoh R, Yamashita T, et al. Development of a time-resolved fluoroimmunoassay(TR-FIA) for testosterone:measurement of serun testosterone concentrations after testosterone treatment in the rainbow trout(Oncorhynchusmykiss)[J]. Gen Comp Endocrinol, 1997; 106(2): 181 - 188.
  • 6[5]Mehto P, Ankelo M, Hinkkanen A, et al.A time-resolved fluoroimmunoassay for the detection of microcystins, cyanobacterial peptede hepa to toxins[J]. Toxin, 2001; 39:831 - 836.
  • 7[6]Uehara M, Lapcik O, Hampl R, et al. Rapid analysis of phytoestrogens in human urine by time-resolved fluoroimmunoassay[J]. Steroid Biochem Mol Biol, 2000; 72(50:273 - 282.
  • 8[8]Ibrahim F, Giton F, Villette JM, et al. Plasma 11 beta-hydroxy- 4-androstene-3, 17-dine: comparison of tied-resolved using tritiated tracer[J]. Steroid Biochem Mol Biol, 2003; 84(5): 563 -568.
  • 9[9]Kobayashi M, Kimura H, Liao J, et al. Measurement of mouse urinary typeⅣ collagen using time-resolved fluoroimmunoassay[J].Anal Sci,2003; 19(2): 205 - 210.
  • 10[10]Fiet J, Giton F, Auzerie J, et al. Development of a new sensitive and specific time-resolved fluoroimmunoassay(TR-FIA) of chlormadinone acetate in the serum of treated menopausal women[J]. Steroids, 2002; 67 ( 13 - 14): 1045 - 1055.

共引文献46

现代医学
2006年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部