摘要
目的:评价大孔吸附树脂纯化葛根、山楂降血脂有效部位的可行性及优选其工艺条件和参数。方法:比较4种大孔吸附树脂对葛根总异黄酮、山楂总黄酮、山楂总三萜酸的吸附性能;以树脂吸附量为指标,对大孔吸附树脂纯化葛根、山楂降血脂有效部位的工艺进行筛选。结果:AB-8大孔吸附树脂对葛根、山楂降血脂有效部位的吸附性能最好,其纯化的最佳条件为原液浓度0·13g生药/ml、流速0·5ml/min、pH值与原液相同(3·5左右)、洗脱剂80%乙醇溶液、洗脱流速2ml/min;纯化后有效部位的纯度达到85%以上。结论:AB-8大孔吸附树脂可用于葛根、山楂降血脂有效部位的纯化,且工艺可行,树脂再生容易。
OBJECTIVE： To study the feasibility of purifying the effective blood - fat dropping parts from radix puerariae and fructus crataegi and to optimize the technical conditions and parameters.METHODS：Absorption capacities of four different macroporous resins on pueraria total isoflavones, crataegi total flavonoids and crataegi total triterpenic acid were compared. With adsorption quantity as index, optimization of the technics of purifying the effective blood - fat dropping parts from radix puerariae and fructus crataegi with macroporous resins were performed. RESULTS：AB- 8 resin showed a best adsorbability on the effective blood - fat dropping parts from radix puerariae and fructus crataegi, its optimum purification condition was the following, the concentration of the stock solution was 0.13g crude drug/ml with a flow rate at 0.5ml/min,and its pH value at about 3.5（ the same as the stock solution）; 80% ethanol solution was taken as the eluting solvent at an eluting flow rate of 2ml/ min.The purity coefficient of the purified effective portions was above 85% .CONCLUSIONS：AB- 8 macroporous resin could be used for the purification of the effective blood - fat dropping parts from radix puerariae and fructus crataegi, the process is feasible and the reproduction of resin is easy.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第11期875-877,共3页
China Pharmacy
基金
江苏省中医药管理局资助项目
关键词
葛根
山楂
大孔树脂
正交试验
纯化
Radix Puerariae
Fructus Crataegi
Macroporous resin
Orthogonal test
Purl fication
