摘要
目的探讨重组人生长激素（r-hGH）对人乳腺癌细胞株MCF-7（MCF-7）有无促进增殖和分化作用,以及r-hGH与5-氟尿嘧啶（5-FU）联合应用对MCF-7的化疗协调作用.方法以不同浓度r-hGH,以及不同浓度的r-hGH联合不同浓度的5-FU分别加入处于对数生长期的MCF-7中进行体外培养,48 h后流式细胞仪检测细胞周期的变化,与对照组进行比较.结果①不同浓度的r-hGH与MCF-7作用后,其细胞周期的G0～G1期、S期、G2～M期的细胞百分比变化,差异均无统计学意义（P＞0.05）;②不同浓度的5-FU与MCF-7作用后,其细胞周期的G0～G1期细胞百分比显著增高（P＜0.05）,S期显著下降（P＜0.01）,G2～M期差异无统计学意义（P＞0.05）;③不同浓度的r-hGH联合不同浓度的5-FU与MCF-7作用后,其细胞周期的G0～G1期细胞百分比显著增高（P＜0.01）,S期和G2～M显著降低（P＜0.01,P＜0.05）.结论单独应用r-hGH对MCF-7的细胞周期无明显影响;r-hGH与5-FU联合应用对MCF-7的化疗具有协调作用,r-hGH能增强5-FU对MCF-7的化疗效果.
Objective To study effect of recombinant human growth hormone （r-hGH） on cell cycle of a human breast eancer cell line MCF-7 , and study the effect of r-hGH plus 5-FU on the effect of chemotherapy in MCF-7.Methods MCF-7 cells were cultured for 48h in RPMI 1640 with r-hGH for different concentrations and with 5-FU and r-hGH for different concentrations, then cells were gathered and evaluated for cell cycle by flow cytometry （FCM） .Results ①Compared with the control group, r-hGH had no significant effect on the percentage of G0-G1, S and G2-M phase of MCF-7 （P 〉0.05）. ②Addition of 5-FU to the culture raised the percentage of G0-G1 phase significantly （P〈0.01） and reduced S phase signifieantly （P〈0.05）, and did not change the percentage of G2-M phase signifieanfly （P〉0.05）. ③Compared with the control group ,5-FU plus r-hGH raised the percentage of G0-G1 phase significantly （P〈0.01） and reduced S , G2-M phase signifieantly （P〈0.05）.Conclusion The r-hGH alone might not have effect on the cell cycle of MCF-7 cells. In vitro r-hGH might promote the sensitivity of chemotherapy of 5-FU to MCF-7 cells.
出处
《广西医学》
CAS
2006年第4期492-495,共4页
Guangxi Medical Journal
基金
广西壮族自治区教育厅科研课题项目（合同号：2001.3.02-097-4）
