摘要
目的探讨小鼠皮内接种H37Ra后,细菌在体内的定植及机体能否产生针对结核菌的免疫力。方法用H37Ra皮内免疫小鼠后15、30、60 d,检测结核菌在小鼠体内脏器的定植;免疫后第30、60天,MTT法检测小鼠脾淋巴细胞受PPD刺激后的增殖转化刺激指数、ELISA法检测小鼠脾淋巴细胞受PPD刺激后IL-2、sIL-2R的表达量。结果小鼠脾、肺脏中有结核菌定植,并至少存活60 d;脾淋巴细胞30、60 d的刺激指数分别为(2.81±0.63)、(2.16±0.52),与BCG皮内接种组无显著性差异,与未免疫组有显著性差异(P<0.05);脾淋巴细胞IL-2、sIL-2R表达量分别为(126.88±8.11)、(91.26±7.29)pg/m l和(138.58±7.67)、(127.09±5.47)pg/m l,与BCG皮内接种组、未免疫组比较均有显著性差异(P<0.05)。结论H37Ra免疫小鼠后能诱导机体产生特异性的抗结核免疫力,且免疫效果略优于BCG。
Objective To study the location of mycobacterium tuberculosis （MTB） and whether the cellular immunity is induced after immunization of H37Ra in mice. Methods Totally 72 BALB/C mice were ineluded. Thirty mice were intraeutaneously injected of 0. 1 ml H37Ra solution （about 10^6 bacteria） at caudal region ; thirty mice were injected of BCG of same quantity instead ; twelve were free from immunization as control. Viable MTB were detected in spleen and lungs on day 15, 30 and 60 after intracutaneous vaccination of H37Ra. The proliferation of T lymphocytes stimulated with purified protein derivative （PPD） was measured by MTr assay and the production of interleukin-2 （IL-2） and soluble interleukin-2 receptor （sIL-2R） in the cultural supernatants of T lymphocytes was also determined by ELISA on day 30 and 60 after intracutaneous vaccination of H37Ra. Rosults Viable H37Ra or BCG could be located in the spleen and lungs in mice for at least 60 d. After H37Ra immunization, the stimulation index （SI） of T lymphocyte proliferation on day 30 and 60 was （2. 81 ± 0. 63 ）, （2. 16 ± 0. 52 ） respectively, which was of no significant difference with that of BCG immunization, but of significant difference with that of non-immunized mice （ P 〈 0. 05 ）. The production of IL-2 and sIL-2R in the H37Ra immunized mice on day 30 and 60 was （ 126.88 ±8. 11 ）, （91.26 ±7.29） and （ 138.58 ±7.67）, （127.09 ±5.47） pg/ml respectively, both higher than BCG immunized mice and non-immunized mice （ P 〈 0. 05 ）. Conclusion H37Ra immunization can induce cellular immunity in mice and the immunity of H37Ra is slightly better than BCG.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第11期1195-1197,共3页
Journal of Third Military Medical University
基金
重庆市教委科研基金资助项目(2004)
重庆医科大学科技创新基金资助项目(CX200204)~~
