摘要
目的观察薏苡仁多糖对实验性糖尿病血管并发症大鼠主动脉内皮素1mRNA表达调控的影响。方法采用链脲佐菌素腹腔注射(75mg/kg)和高热量饲料喂养建立2型糖尿病并发动脉粥样硬化大鼠模型,应用RT-PCR半定量分析大鼠主动脉内皮素1mRNA的表达变化。结果模型组大鼠主动脉内皮素1mRNA表达量为0.72±0.10,明显高于正常组的0.39±0.01(P<0.01),经给药处理6个月后,与模型组的表达量比较,各给药组大鼠主动脉内皮素1mRNA有不同程度的下调(P<0.05),其中薏苡仁多糖注射组的表达量0.49±0.12与模型对照组比较有非常显著性差异(P<0.01)。结论糖尿病血管并发症的发生可能与内皮素1mRNA表达上调有关,薏苡仁多糖保护糖尿病血管内皮损伤可能与其下调内皮素1mRNA表达的作用相关。
Aim To explore the effect of coixan on endothehum-1 （ET-1） gene expression of type 2 diabetic model on rat arota. Methods The experimental type 2 diabetic rats were yielded by i. p streptozotoein （75 mg/kg） and feeding hyperotrophic foods, then to observe ET-1 gene expression by RT-PCR. Results After 6 months of i. p streptozotoein, the ET-1 gene expression 0.72 ± 0.10 on rat arotic enhanced obviously, the cocnparison between normal group 0.39 ±0.01 （ P〈0.01 ）, ET-1 mRNA gene expression of administered groups dowrtregulate （P〈0.05）, and the ET-1 gene expression of coixan i. p group 0.49 ±0.12 showed mere significant difference than the model control group （ P〈0.01）. Conclusion Diabetic vascular complication may be related to upregulation of ET-1 mRNA gene expression,Coixan decreased ET-1 mRNA gene expression.
出处
《中国动脉硬化杂志》
CAS
CSCD
2006年第3期194-196,共3页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金资助(30200362)
