摘要
目的比较单纯的、添加锌锶等元素及复合rhBMP-2的新型可注射可降解磷酸钙骨水泥(CPC)植入机体后的降解及成骨作用,检验材料及其改进剂型在临床应用的可能性。方法将24只雄性新西兰白兔随机分为A,B,C三组,分别为9、9和6只,制备双侧胫骨结节骨缺损模型；于骨缺损处分别置入：A组为单纯新型可注射可降解CPC材料,B组为含锌锶等微量元素的该CPC材料,C组为该CPC材料与rhBMP-2的复合物。观察其全身及植入局部反应,术后4、8、16周取材CT扫描、肉眼及光学显微镜观察材料植入后的局部反应情况并对比观察该三种材料的降解及骨生长情况。组织切片摄像后,每组每时间点各取5幅相片用软件进行图文处理,求得术后骨组织切片中骨组织含量百分比。结果CT扫描、肉眼及光学显微镜观察发现单纯的和含锌锶等微量元素的CPC材料可引导新骨形成,但新骨形成少且慢,与材料降解不同步；复合rhBMP-2的CPC材料组新生骨形成多而早,基本与材料降解同步。术后骨组织含量百分比测定,A组为(41．7±16．6)%,B组为(31．2±12．2)%,C组为(71．7±21．0)%,复合BMP的CPC材料组术后骨组织切片中骨组织含量显著高于另外两组(P＜0．01)。结论复合rhBMP-2的可注射可降解CPC材料可较早地诱导新生骨生成,与单纯的及含锌锶等微量元素的可注射可降解CPC材料相比,更适合于临床使用。
Objective To compare the osseo-induction and biodegradation performances of three types of injectable and degradable calcium phosphate cement （CPC） so as to find out a better bone substitute. Methods Three types of injectable and degradable CPC were respectively implanted into the bilateral tibias of 24 New Zealand rabbits： pure CPC （Group A）, CPC added with Zinc and Strontiumions （Group B）, and CPC with composite rhBMP-2 （Group C） . Their systematic and local reactions in implanted region were closely observed. The degradation and osseo-induction performances were compared macroscopically, microscopically and by CT scan to find out the one that could best meet clinical needs. Tissue slices were sampled and photographed four, eight and 16 weeks after operation. Five photographs were selected in each group and at each time points for computer software （Image Pro Plus 5.1 ） processing to calculate the percentages of bone in the images of postoperative slices. Results In Groups A and B, new bone was found to form slowly and little by little, and the ossification was not synchronous with the material degradation. In Group C, however, new bone was observed to form early and massively, and the ossification was almost synchronous with the material degradation. In Groups A, B and C, the percentage of bone in the images of postoperative slices was （41.7 ± 16.6） %, （31.2 ± 12.2） % and （71.7 ± 21.0） % respectively. The bone percentage in CPC with composite rhBMP-2 was significantly higher than that in the other two types of CPC （ P 〈 0.01 ） . Conclusion The injectable and degradable CPC with composite rhBMP-2 is more suitable for clinical use, because it can induce early new bone formation and synchronous biodegradation.
出处
《中华创伤骨科杂志》
CAS
CSCD
2006年第6期557-561,共5页
Chinese Journal of Orthopaedic Trauma
基金
国家自然科学基金项目(011561)
关键词
磷酸钙骨水泥
降解
成骨作用
Calcium phosphate cement （CPC）
Biodegradation
Osseo-induction
